Ultraviolet-B Radiation Induced Cross-linking Improves Physical Properties of Cold- and Warm-Water Fish Gelatin Gels and Films

Authors

  • Caio G. Otoni,

    1. Authors Otoni, Chiou, Bilbao-Sainz, and McHugh are with Agricultural Research Service, Western Regional Research Center, Albany, CA 94710, U.S.A. Author Avena-Bustillos is with Dept. of Biological and Agricultural Engineering, Univ. of California, Davis, CA 95616, U.S.A. Author Bechtel is with Agricultural Research Service, Subarctic Agricultural Research Unit, Fishery Industrial Technology Center, Kodiak, AK 99615, U.S.A. Direct inquiries to author Avena-Bustillos (E-mail : roberto.avena@ars.usda.gov).
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  • Roberto J. Avena-Bustillos,

    1. Authors Otoni, Chiou, Bilbao-Sainz, and McHugh are with Agricultural Research Service, Western Regional Research Center, Albany, CA 94710, U.S.A. Author Avena-Bustillos is with Dept. of Biological and Agricultural Engineering, Univ. of California, Davis, CA 95616, U.S.A. Author Bechtel is with Agricultural Research Service, Subarctic Agricultural Research Unit, Fishery Industrial Technology Center, Kodiak, AK 99615, U.S.A. Direct inquiries to author Avena-Bustillos (E-mail : roberto.avena@ars.usda.gov).
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  • Bor-Sen Chiou,

    1. Authors Otoni, Chiou, Bilbao-Sainz, and McHugh are with Agricultural Research Service, Western Regional Research Center, Albany, CA 94710, U.S.A. Author Avena-Bustillos is with Dept. of Biological and Agricultural Engineering, Univ. of California, Davis, CA 95616, U.S.A. Author Bechtel is with Agricultural Research Service, Subarctic Agricultural Research Unit, Fishery Industrial Technology Center, Kodiak, AK 99615, U.S.A. Direct inquiries to author Avena-Bustillos (E-mail : roberto.avena@ars.usda.gov).
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  • Cristina Bilbao-Sainz,

    1. Authors Otoni, Chiou, Bilbao-Sainz, and McHugh are with Agricultural Research Service, Western Regional Research Center, Albany, CA 94710, U.S.A. Author Avena-Bustillos is with Dept. of Biological and Agricultural Engineering, Univ. of California, Davis, CA 95616, U.S.A. Author Bechtel is with Agricultural Research Service, Subarctic Agricultural Research Unit, Fishery Industrial Technology Center, Kodiak, AK 99615, U.S.A. Direct inquiries to author Avena-Bustillos (E-mail : roberto.avena@ars.usda.gov).
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  • Peter J. Bechtel,

    1. Authors Otoni, Chiou, Bilbao-Sainz, and McHugh are with Agricultural Research Service, Western Regional Research Center, Albany, CA 94710, U.S.A. Author Avena-Bustillos is with Dept. of Biological and Agricultural Engineering, Univ. of California, Davis, CA 95616, U.S.A. Author Bechtel is with Agricultural Research Service, Subarctic Agricultural Research Unit, Fishery Industrial Technology Center, Kodiak, AK 99615, U.S.A. Direct inquiries to author Avena-Bustillos (E-mail : roberto.avena@ars.usda.gov).
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  • Tara H. McHugh

    1. Authors Otoni, Chiou, Bilbao-Sainz, and McHugh are with Agricultural Research Service, Western Regional Research Center, Albany, CA 94710, U.S.A. Author Avena-Bustillos is with Dept. of Biological and Agricultural Engineering, Univ. of California, Davis, CA 95616, U.S.A. Author Bechtel is with Agricultural Research Service, Subarctic Agricultural Research Unit, Fishery Industrial Technology Center, Kodiak, AK 99615, U.S.A. Direct inquiries to author Avena-Bustillos (E-mail : roberto.avena@ars.usda.gov).
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Abstract

Abstract:  Cold- and warm-water fish gelatin granules were exposed to ultraviolet-B radiation for doses up to 29.7 J/cm2. Solutions and films were prepared from the granules. Gel electrophoresis and refractive index were used to examine changes in molecular weight of the samples. Also, the gel strength and rheological properties of the solutions as well as the tensile and water vapor barrier properties of the films were characterized. SDS-PAGE and refractive index results indicated cross-linking of gelatin chains after exposure to radiation. Interestingly, UV-B treated samples displayed higher gel strengths, with cold- and warm-water fish gelatin having gel strength increases from 1.39 to 2.11 N and from 7.15 to 8.34 N, respectively. In addition, both gelatin samples exhibited an increase in viscosity for higher UV doses. For gelatin films, the cold-water fish gelatin samples made from irradiated granules showed greater tensile strength. In comparison, the warm-water gelatin films made from irradiated granules had lower tensile strength, but better water vapor barrier properties. This might be due to the UV induced cross-linking in warm-water gelatin that disrupted helical structures.

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