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Season's Variation Impact on Citrus aurantium Leaves Essential Oil: Chemical Composition and Biological Activities

Authors

  • Inès Ellouze,

    1. Authors Ellouze and Bouajila are with Laboratoire des Interactions Moléculaires et Réactivité Chimique et Photochimique, Univ. Paul Sabatier Toulouse 3, Toulouse, France. Authors Ellouze and Abderrabba are with Unité de Recherche PhysicoChimie Moléculaire, Inst. Préparatoire aux Etudes Scientifiques et Techniques, La Marsa, Tunis, Tunisie. Author Sabaou is with Laboratoire de recherche sur les produits bioactifs et la valorisation de la biomasse, Ecole Normale Supérieure de Kouba, Alger, Algérie. Authors Mathieu and Lebrihi are with Laboratoire de Génie chimiques, ENSAT-INP, Toulouse, France. Direct inquiries to author Bouajila (E-mail: bouajila@cict.fr) and Abderraba (E-mail: manef@)ipest.rnu.tn).
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  • Manef Abderrabba,

    1. Authors Ellouze and Bouajila are with Laboratoire des Interactions Moléculaires et Réactivité Chimique et Photochimique, Univ. Paul Sabatier Toulouse 3, Toulouse, France. Authors Ellouze and Abderrabba are with Unité de Recherche PhysicoChimie Moléculaire, Inst. Préparatoire aux Etudes Scientifiques et Techniques, La Marsa, Tunis, Tunisie. Author Sabaou is with Laboratoire de recherche sur les produits bioactifs et la valorisation de la biomasse, Ecole Normale Supérieure de Kouba, Alger, Algérie. Authors Mathieu and Lebrihi are with Laboratoire de Génie chimiques, ENSAT-INP, Toulouse, France. Direct inquiries to author Bouajila (E-mail: bouajila@cict.fr) and Abderraba (E-mail: manef@)ipest.rnu.tn).
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  • Nassereddine Sabaou,

    1. Authors Ellouze and Bouajila are with Laboratoire des Interactions Moléculaires et Réactivité Chimique et Photochimique, Univ. Paul Sabatier Toulouse 3, Toulouse, France. Authors Ellouze and Abderrabba are with Unité de Recherche PhysicoChimie Moléculaire, Inst. Préparatoire aux Etudes Scientifiques et Techniques, La Marsa, Tunis, Tunisie. Author Sabaou is with Laboratoire de recherche sur les produits bioactifs et la valorisation de la biomasse, Ecole Normale Supérieure de Kouba, Alger, Algérie. Authors Mathieu and Lebrihi are with Laboratoire de Génie chimiques, ENSAT-INP, Toulouse, France. Direct inquiries to author Bouajila (E-mail: bouajila@cict.fr) and Abderraba (E-mail: manef@)ipest.rnu.tn).
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  • Florence Mathieu,

    1. Authors Ellouze and Bouajila are with Laboratoire des Interactions Moléculaires et Réactivité Chimique et Photochimique, Univ. Paul Sabatier Toulouse 3, Toulouse, France. Authors Ellouze and Abderrabba are with Unité de Recherche PhysicoChimie Moléculaire, Inst. Préparatoire aux Etudes Scientifiques et Techniques, La Marsa, Tunis, Tunisie. Author Sabaou is with Laboratoire de recherche sur les produits bioactifs et la valorisation de la biomasse, Ecole Normale Supérieure de Kouba, Alger, Algérie. Authors Mathieu and Lebrihi are with Laboratoire de Génie chimiques, ENSAT-INP, Toulouse, France. Direct inquiries to author Bouajila (E-mail: bouajila@cict.fr) and Abderraba (E-mail: manef@)ipest.rnu.tn).
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  • Ahmed Lebrihi,

    1. Authors Ellouze and Bouajila are with Laboratoire des Interactions Moléculaires et Réactivité Chimique et Photochimique, Univ. Paul Sabatier Toulouse 3, Toulouse, France. Authors Ellouze and Abderrabba are with Unité de Recherche PhysicoChimie Moléculaire, Inst. Préparatoire aux Etudes Scientifiques et Techniques, La Marsa, Tunis, Tunisie. Author Sabaou is with Laboratoire de recherche sur les produits bioactifs et la valorisation de la biomasse, Ecole Normale Supérieure de Kouba, Alger, Algérie. Authors Mathieu and Lebrihi are with Laboratoire de Génie chimiques, ENSAT-INP, Toulouse, France. Direct inquiries to author Bouajila (E-mail: bouajila@cict.fr) and Abderraba (E-mail: manef@)ipest.rnu.tn).
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  • Jalloul Bouajila

    1. Authors Ellouze and Bouajila are with Laboratoire des Interactions Moléculaires et Réactivité Chimique et Photochimique, Univ. Paul Sabatier Toulouse 3, Toulouse, France. Authors Ellouze and Abderrabba are with Unité de Recherche PhysicoChimie Moléculaire, Inst. Préparatoire aux Etudes Scientifiques et Techniques, La Marsa, Tunis, Tunisie. Author Sabaou is with Laboratoire de recherche sur les produits bioactifs et la valorisation de la biomasse, Ecole Normale Supérieure de Kouba, Alger, Algérie. Authors Mathieu and Lebrihi are with Laboratoire de Génie chimiques, ENSAT-INP, Toulouse, France. Direct inquiries to author Bouajila (E-mail: bouajila@cict.fr) and Abderraba (E-mail: manef@)ipest.rnu.tn).
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Abstract

Abstract:  Citrus aurantium leaves' essential oils (EOs) were evaluated for chemical composition and antioxidant and antibacterial activities. The vegetable material, taken 5 times during the year, has undergone the hydrodistillation to prepare EO. Chemical characterization by gas chromatography/mass spectrometry and GC/flame ionization detection allowed the identification of 46 compounds, and a notable quantitative and qualitative differences between the different Petitgrain samples according to the harvest time. Linalool (43.2% to 65.97%), linalyl acetate (0.77% to 24.77%), and α-terpineol (9.29% to 12.12%) were the main components. The most important number of components was registered for summer EOs (July and September). The 5 EOs submitted biological activities screening, namely, antioxidant and antimicrobial activities. Weak antioxidant activities (IC50 values >10000 mg/L) were registered by both 1,1-diphenyl-2-picrylhydrazyl and 2,2′-azinobis-3-ethylbenzothiazoline-6-sulphonate assays, mostly because the weak amount of phenols in EOs. Antibacterial activities (12 microorganisms) were registered against Gram-positive bacteria [Bacillus subtilis (MIC = 2.7 mg/mL), Staphylococcus aureus (4.8 mg/mL)], and moderated ones against yeasts [Saccharomyces cerevisiae (9.2 mg/mL)] and fungi [Mucor ramannianus (5 mg/mL)]. Positive correlations between the identified compounds and the antimicrobial activities were noted. Many compounds were correlated to antimicrobial activity mainly caryophyllene oxide against Escherichia coli (R2= 0.99), S. cerevisiae (R2= 0.99), and Fusarium culmorum (R2= 0.99).

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