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Occurrence and Antimicrobial Susceptibility of Listeria monocytogenes Isolated from Brined White Cheese in Jordan

Authors

  • Tareq M. Osaili,

    1. Authors Osaili, Al-Nabulsi, Taha, and Shaker are with Dept. of Nutrition and Food Technology, Faculty of Agriculture, Jordan Univ. of Science and Technology, Irbid, 22110 Jordan. Author Al-Holy is with Dept. of Clinical Nutrition and Dietetics, Faculty of Allied Health Sciences, Hashemite Univ., Zarqa, 13115 Jordan. Author Alaboudi is with Dept. of Pathology and Animal Health, Faculty of Veterinary Medicine, Jordan Univ. of Science and Technology, Irbid, 22110 Jordan. Author Al-Rousan is with Dept. of Applied Sciences, Al-Huson Univ. College, Al-Balqa’ Applied Univ., Irbid, Jordan. Direct inquiries to author Osaili (E-mail: tosaili@just.edu.jo).
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  • Anas A. Al-Nabulsi,

    1. Authors Osaili, Al-Nabulsi, Taha, and Shaker are with Dept. of Nutrition and Food Technology, Faculty of Agriculture, Jordan Univ. of Science and Technology, Irbid, 22110 Jordan. Author Al-Holy is with Dept. of Clinical Nutrition and Dietetics, Faculty of Allied Health Sciences, Hashemite Univ., Zarqa, 13115 Jordan. Author Alaboudi is with Dept. of Pathology and Animal Health, Faculty of Veterinary Medicine, Jordan Univ. of Science and Technology, Irbid, 22110 Jordan. Author Al-Rousan is with Dept. of Applied Sciences, Al-Huson Univ. College, Al-Balqa’ Applied Univ., Irbid, Jordan. Direct inquiries to author Osaili (E-mail: tosaili@just.edu.jo).
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  • Mohammad H. Taha,

    1. Authors Osaili, Al-Nabulsi, Taha, and Shaker are with Dept. of Nutrition and Food Technology, Faculty of Agriculture, Jordan Univ. of Science and Technology, Irbid, 22110 Jordan. Author Al-Holy is with Dept. of Clinical Nutrition and Dietetics, Faculty of Allied Health Sciences, Hashemite Univ., Zarqa, 13115 Jordan. Author Alaboudi is with Dept. of Pathology and Animal Health, Faculty of Veterinary Medicine, Jordan Univ. of Science and Technology, Irbid, 22110 Jordan. Author Al-Rousan is with Dept. of Applied Sciences, Al-Huson Univ. College, Al-Balqa’ Applied Univ., Irbid, Jordan. Direct inquiries to author Osaili (E-mail: tosaili@just.edu.jo).
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  • Murad A. Al-Holy,

    1. Authors Osaili, Al-Nabulsi, Taha, and Shaker are with Dept. of Nutrition and Food Technology, Faculty of Agriculture, Jordan Univ. of Science and Technology, Irbid, 22110 Jordan. Author Al-Holy is with Dept. of Clinical Nutrition and Dietetics, Faculty of Allied Health Sciences, Hashemite Univ., Zarqa, 13115 Jordan. Author Alaboudi is with Dept. of Pathology and Animal Health, Faculty of Veterinary Medicine, Jordan Univ. of Science and Technology, Irbid, 22110 Jordan. Author Al-Rousan is with Dept. of Applied Sciences, Al-Huson Univ. College, Al-Balqa’ Applied Univ., Irbid, Jordan. Direct inquiries to author Osaili (E-mail: tosaili@just.edu.jo).
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  • Akram R. Alaboudi,

    1. Authors Osaili, Al-Nabulsi, Taha, and Shaker are with Dept. of Nutrition and Food Technology, Faculty of Agriculture, Jordan Univ. of Science and Technology, Irbid, 22110 Jordan. Author Al-Holy is with Dept. of Clinical Nutrition and Dietetics, Faculty of Allied Health Sciences, Hashemite Univ., Zarqa, 13115 Jordan. Author Alaboudi is with Dept. of Pathology and Animal Health, Faculty of Veterinary Medicine, Jordan Univ. of Science and Technology, Irbid, 22110 Jordan. Author Al-Rousan is with Dept. of Applied Sciences, Al-Huson Univ. College, Al-Balqa’ Applied Univ., Irbid, Jordan. Direct inquiries to author Osaili (E-mail: tosaili@just.edu.jo).
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  • Walid M. Al-Rousan,

    1. Authors Osaili, Al-Nabulsi, Taha, and Shaker are with Dept. of Nutrition and Food Technology, Faculty of Agriculture, Jordan Univ. of Science and Technology, Irbid, 22110 Jordan. Author Al-Holy is with Dept. of Clinical Nutrition and Dietetics, Faculty of Allied Health Sciences, Hashemite Univ., Zarqa, 13115 Jordan. Author Alaboudi is with Dept. of Pathology and Animal Health, Faculty of Veterinary Medicine, Jordan Univ. of Science and Technology, Irbid, 22110 Jordan. Author Al-Rousan is with Dept. of Applied Sciences, Al-Huson Univ. College, Al-Balqa’ Applied Univ., Irbid, Jordan. Direct inquiries to author Osaili (E-mail: tosaili@just.edu.jo).
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  • Reyad R. Shaker

    1. Authors Osaili, Al-Nabulsi, Taha, and Shaker are with Dept. of Nutrition and Food Technology, Faculty of Agriculture, Jordan Univ. of Science and Technology, Irbid, 22110 Jordan. Author Al-Holy is with Dept. of Clinical Nutrition and Dietetics, Faculty of Allied Health Sciences, Hashemite Univ., Zarqa, 13115 Jordan. Author Alaboudi is with Dept. of Pathology and Animal Health, Faculty of Veterinary Medicine, Jordan Univ. of Science and Technology, Irbid, 22110 Jordan. Author Al-Rousan is with Dept. of Applied Sciences, Al-Huson Univ. College, Al-Balqa’ Applied Univ., Irbid, Jordan. Direct inquiries to author Osaili (E-mail: tosaili@just.edu.jo).
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Abstract

Abstract:  Listeria monocytogenes is a serious foodborne pathogen that has been isolated from different dairy food products. Several foodborne outbreaks of listeriosis have been associated with consumption of cheese. The aims of this study were to determine the occurrence of L. monocytogenes and Listeria spp. in brined white cheese (BWC) sold in Jordan, and to determine the susceptibility of isolated L. monocytogenes to antimicrobials. Three hundred and fifty samples of 5 different types of BWC (akkawi, boiled, halloumi, pasteurized, and shellal) were collected from a local market in Jordan. The ISO (11290-1) procedure was followed for isolation and identification of Listeria spp. from cheese samples and a polymerase chain reaction (PCR) technique was used for confirmation of L. monocytogenes isolates. The VITEK2 automated system was used for testing antimicrobial susceptibility of L. monocytogenes isolates. The overall prevalence of Listeria spp. in cheese sample was 27.1%. L. monocytogenes was isolated from 39 (11.1%) samples. Other isolated species were L. grayi (6.9%), L. innocua (2%), L. ivanovii (4%), L. seeligeri (2%), and L. welshimeri (0.3%). The pH values and salt concentrations of L. monocytogenes positive cheese samples ranged from 5.10 to 6.32 and 5.64 to 13.16, respectively. L. monocytogenes isolates were sensitive or intermediate susceptible to imipenem, gentamicin, linezolid, teicoplanin, vancomycin, fusidic acid, trimethoprim/sulfamethoxazole, benzylpenicillin, ciprofloxacin, erythromycin, tetracycline, and rifampicin, but resistant to fosfomycin, oxacillin, and clindamycin.

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