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Microwave-Assisted Extraction and Determination of Citrus Red 2 Dye in Oranges and Orange Juice by Liquid Chromatography-Tandem Mass Spectrometry

Authors

  • Chao Han,

    1. Authors Han, Liu, Zhu, Huang, and Chen are with Wenzhou Entry-Exit Inspection and Quarantine Bureau of P.R.C, Wenzhou, 325027, China and author Shen is with College of Chemistry and Materials Engineering, Wenzhou Univ., Wenzhou, 325035, China. Direct inquiries to author Shen (E-mail: lwsheny@yahoo.com.cn).
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  • Bin Liu,

    1. Authors Han, Liu, Zhu, Huang, and Chen are with Wenzhou Entry-Exit Inspection and Quarantine Bureau of P.R.C, Wenzhou, 325027, China and author Shen is with College of Chemistry and Materials Engineering, Wenzhou Univ., Wenzhou, 325035, China. Direct inquiries to author Shen (E-mail: lwsheny@yahoo.com.cn).
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  • Zhenou Zhu,

    1. Authors Han, Liu, Zhu, Huang, and Chen are with Wenzhou Entry-Exit Inspection and Quarantine Bureau of P.R.C, Wenzhou, 325027, China and author Shen is with College of Chemistry and Materials Engineering, Wenzhou Univ., Wenzhou, 325035, China. Direct inquiries to author Shen (E-mail: lwsheny@yahoo.com.cn).
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  • Fuzhen Huang,

    1. Authors Han, Liu, Zhu, Huang, and Chen are with Wenzhou Entry-Exit Inspection and Quarantine Bureau of P.R.C, Wenzhou, 325027, China and author Shen is with College of Chemistry and Materials Engineering, Wenzhou Univ., Wenzhou, 325035, China. Direct inquiries to author Shen (E-mail: lwsheny@yahoo.com.cn).
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  • Xiangzhun Chen,

    1. Authors Han, Liu, Zhu, Huang, and Chen are with Wenzhou Entry-Exit Inspection and Quarantine Bureau of P.R.C, Wenzhou, 325027, China and author Shen is with College of Chemistry and Materials Engineering, Wenzhou Univ., Wenzhou, 325035, China. Direct inquiries to author Shen (E-mail: lwsheny@yahoo.com.cn).
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  • Yan Shen

    1. Authors Han, Liu, Zhu, Huang, and Chen are with Wenzhou Entry-Exit Inspection and Quarantine Bureau of P.R.C, Wenzhou, 325027, China and author Shen is with College of Chemistry and Materials Engineering, Wenzhou Univ., Wenzhou, 325035, China. Direct inquiries to author Shen (E-mail: lwsheny@yahoo.com.cn).
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Abstract

Abstract:  A fast, simple, low cost, and high-throughput method has been developed for the determination of citrus red 2 dye in orange and orange juice samples. The procedure is based on microwave-assisted extraction followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. The method was optimized, and the analyte was efficiently extracted from the samples in 30 min using hexane/acetone (v/v, 3 : 1). The method was validated and showed good linearity and selectivity. The limits of quantification (LOQs) were 5 μg/kg (sample size of 2 g) for both orange and orange juice samples. The average recoveries, measured at 3 concentration levels (5, 10, and 20 μg/kg), were in the range 77.5% to 87.6% for the compound tested with relative standard deviations below 7.3%. The proposed method is rapid, accurate, and could be utilized for the routine analysis of citrus red 2 dye in orange and orange juice samples.

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