Get access

The Beneficial Effect of Folate-Enriched Egg on the Folate and Homocysteine Levels in Rats Fed a Folate- and Choline-Deficient Diet

Authors

  • Ayami Sugiyama,

    1. Authors Ayami Sugiyama, Kenji Horie and Mujo Kim are with Pharma Foods Intl. Co. Ltd.1-49, Goryo-Ohara, Nishikyo-ku, Kyoto, 615–8245, Japan. Authors Hiroyo Awaji and Rieko Nakata are with Dept. of Food Science and Nutrition, Nara Women's Univ., Nara, 630- 8506, Japan. Direct inquiries to author Rieko Nakata (E-mail: r-nakata@cc.nara-wu.ac.jp).
    Search for more papers by this author
  • Hiroyo Awaji,

    1. Authors Ayami Sugiyama, Kenji Horie and Mujo Kim are with Pharma Foods Intl. Co. Ltd.1-49, Goryo-Ohara, Nishikyo-ku, Kyoto, 615–8245, Japan. Authors Hiroyo Awaji and Rieko Nakata are with Dept. of Food Science and Nutrition, Nara Women's Univ., Nara, 630- 8506, Japan. Direct inquiries to author Rieko Nakata (E-mail: r-nakata@cc.nara-wu.ac.jp).
    Search for more papers by this author
  • Kenji Horie,

    1. Authors Ayami Sugiyama, Kenji Horie and Mujo Kim are with Pharma Foods Intl. Co. Ltd.1-49, Goryo-Ohara, Nishikyo-ku, Kyoto, 615–8245, Japan. Authors Hiroyo Awaji and Rieko Nakata are with Dept. of Food Science and Nutrition, Nara Women's Univ., Nara, 630- 8506, Japan. Direct inquiries to author Rieko Nakata (E-mail: r-nakata@cc.nara-wu.ac.jp).
    Search for more papers by this author
  • Mujo Kim,

    1. Authors Ayami Sugiyama, Kenji Horie and Mujo Kim are with Pharma Foods Intl. Co. Ltd.1-49, Goryo-Ohara, Nishikyo-ku, Kyoto, 615–8245, Japan. Authors Hiroyo Awaji and Rieko Nakata are with Dept. of Food Science and Nutrition, Nara Women's Univ., Nara, 630- 8506, Japan. Direct inquiries to author Rieko Nakata (E-mail: r-nakata@cc.nara-wu.ac.jp).
    Search for more papers by this author
  • Rieko Nakata

    1. Authors Ayami Sugiyama, Kenji Horie and Mujo Kim are with Pharma Foods Intl. Co. Ltd.1-49, Goryo-Ohara, Nishikyo-ku, Kyoto, 615–8245, Japan. Authors Hiroyo Awaji and Rieko Nakata are with Dept. of Food Science and Nutrition, Nara Women's Univ., Nara, 630- 8506, Japan. Direct inquiries to author Rieko Nakata (E-mail: r-nakata@cc.nara-wu.ac.jp).
    Search for more papers by this author

Abstract

Abstract:  We investigated the effects of folate-enriched egg yolk powder on folate and homocysteine levels in plasma and liver of rats fed the folate- and choline-deficient diet to determine bioavailability in vivo. Three-wk-old Wistar rats were fed (1) the pteroylglutamate (PteGlu), (2) the choline, (3) the PteGlu and choline, (4) the folate-enriched egg yolk powder diet for 4 wk after having been fed the folate- and choline-deficient diet. The hepatic folate level in the folate-enriched egg yolk powder group was significantly higher than that in the folate- and choline-deficient or the control groups. The homocysteine concentration in plasma and liver of the folate-enriched egg yolk powder group was significantly lower than that of the folate- and choline-deficient or the PteGlu groups. The S-adenosyl-methionine (SAM)/S-adenosyl-homocysteine (SAH) ratio in the folate-enriched egg yolk powder group was significantly higher than that in the folate- and choline-deficient group. These effects were similar in the PteGlu and choline, but not the PteGlu or the choline groups. These data suggest that the intake of folate-enriched eggs, as well as of both folate and choline, induced the beneficial effects on folate and homocysteine metabolism. Thus, folate-enriched eggs could be used as beneficial source of folate with a high bioavailability.

Ancillary