Evaluation of a PCR assay for identification and differentiation of Campylobacter fetus subspecies
Article first published online: 10 MAR 2008
Australian Veterinary Journal
Volume 75, Issue 11, pages 827–831, November 1997
How to Cite
HUM, S., QUINN, K., BRUNNER, J. and ON, S. (1997), Evaluation of a PCR assay for identification and differentiation of Campylobacter fetus subspecies. Australian Veterinary Journal, 75: 827–831. doi: 10.1111/j.1751-0813.1997.tb15665.x
- Issue published online: 10 MAR 2008
- Article first published online: 10 MAR 2008
- Accepted for publication 13 June 1997
- Campylobacter fetus;
- macrorestriction profiling
Objective To evaluate a polymerase chain reaction assay for identification of Campylobacter fetus and differentiation of the defined subspecies.
Design Characterisation of bacterial strains by traditional phenotyping, polymerase chain reaction, a probabilistic identification scheme and macrorestriction profiling using pulsed field gel electrophoresis.
Procedure The results of identification of 99 bacterial strains as determined by conventional phenotyping or by poly-merase chain reaction were compared. Two of these were type strains of C fetus subsp fetus and C fetus subsp venerealis; the remaining strains were field isolates putatively identified as C fetus. In cases where the subspecies identity was disputed, isolates were identified by means of a probabilistic identification scheme and by macrorestriction profiling.
Results The agreement between strain identities initially suggested by traditional phenotypic methods and the PCR assay was found to be 80.8%. The polymerase chain reaction proved to be a reliable technique for the species and subspecies identification of C fetus; equivocal results were obtained in only two instances. Initial misidentifications by conventional phenotyping methods were attributed to methodological differences used in various laboratories.
Conclusion Our results indicate that misidentification of C fetus i n routine diagnostic laboratories may be relatively common. The PCR assay evaluated gave rapid and reproducible results and is thus a valuable adjunctive method for the identification of C fetus and subsequent subspecies differentiation.