Using 20-gauge percutaneous peripheral catheters to reliably collect serial 4-mL blood samples from conscious dogs
Article first published online: 24 MAY 2010
© 2010 The Authors. Journal compilation © 2010 Australian Veterinary Association
Australian Veterinary Journal
Volume 88, Issue 6, pages 215–221, June 2010
How to Cite
Elliott, K., Fleeman, L. and Rand, J. (2010), Using 20-gauge percutaneous peripheral catheters to reliably collect serial 4-mL blood samples from conscious dogs. Australian Veterinary Journal, 88: 215–221. doi: 10.1111/j.1751-0813.2010.00580.x
- Issue published online: 24 MAY 2010
- Article first published online: 24 MAY 2010
- (Accepted for publication 23 October 2009)
- cephalic vein;
- serial blood samples
Objective To determine the reliability of collecting serial 4-mL blood samples over 13 h using a 20-gauge, cephalic catheter in conscious dogs.
Design Prospective, observational study.
Animals Twelve (6 males, 6 females) healthy, neutered, lean dogs.
Procedure Percutaneous placement of a 20-gauge, 1.1 × 30 mm intravenous over-the-needle catheter into the cephalic vein was performed in each dog on three occasions. Catheter patency was maintained by intermittent flushing with 0.9% saline and 1 IU/mL heparinised saline solutions. Blood samples (4 mL) were obtained at 10 time-points over 13 h, with close monitoring of the dogs and the indwelling catheters. Blood volume, resistance on aspirating blood sample (minimal or marked resistance) and the methods used to improve blood flow were recorded.
Results A high proportion (93%) of blood sample collections achieved the required 4-mL volume, and the remaining samples were greater than 1.5 mL. Of the 358 collections via the cephalic catheter, 311 (87%) were obtained with ‘minimal resistance’. The remaining collections had ‘marked resistance’ (n=47) and were associated with a loose catheter in 11% (5/47) and of unknown cause in 89% (42/47). After ‘marked resistance’ had been encountered and the catheter was flushed with saline and heparin solutions, subsequent sampling with ‘minimal resistance’ was frequently possible from the same catheter.
Conclusion Use of a percutaneous, 20-gauge intravenous cephalic catheter allowed reliable serial collection of 4-mL blood samples over 13 h in conscious dogs.