*Supported in part by the U.S. Atomic Energy Commission, Division of Biology and Medicine, Contracts AT(30-1)3401 and AT(30-1)2802. Contribution Number 643 of the McCollum-Pratt Institute.
QUANTUM YIELDS OF THE LUMINOL CHEMILUMINESCENCE REACTION IN AQUEOUS AND APROTIC SOLVENTS*
Article first published online: 2 JAN 2008
Photochemistry and Photobiology
Volume 15, Issue 2, pages 227–237, February 1972
How to Cite
LEE, J. and SELIGER, H. H. (1972), QUANTUM YIELDS OF THE LUMINOL CHEMILUMINESCENCE REACTION IN AQUEOUS AND APROTIC SOLVENTS. Photochemistry and Photobiology, 15: 227–237. doi: 10.1111/j.1751-1097.1972.tb06241.x
- Issue published online: 2 JAN 2008
- Article first published online: 2 JAN 2008
- Received 22 March 1971; accepted 2 June 1971
Abstract— Quantum yields for luminol (3-aminophthalic hydrazide) chemiluminescence reactions fall into two classes depending on oxidizing conditions. In aprotic solvents the quantum yield is high and the excitation yield which allows for the fluorescence quantum yield of the product, is 0·09 and is unaffected by changes in solution temperature or polarity, or the presence of quenchers. In aqueous solution under optimum pH conditions (11–13), hydrogen peroxide oxidation results in a high chemiluminescence quantum yield with an excitation yield of 0·04 again unaffected by temperature, viscosity or quenchers. Other oxidizing conditions produce lower quantum yields probably by the introduction of competing chemical pathways.
The luminol chemiluminescence light standard has been used to calibrate a spectrofluoro-meter with results in good agreement with the quantum yields of the ferrioxalate actinometer and the fluorescence of quinine sulfate and diphenylanthracene.