SINGLET MOLECULAR OXYGEN INDUCED MUTAGENICITY IN A MAMMALIAN SV40-BASED SHUTTLE VECTOR

Authors

  • P. Di Mascio,

    1. Institut fur Physiologische Chemie I, University of Dusseldorf, Moorenstr. 5, D-4000 Dusseldorf, W. Germany, department of Biology, Instituto de Biociencias, CP 11461, Universidade de Sao Paulo, Sao Paulo, 05499, SP, Brazil
    Search for more papers by this author
  • C. F. M. Menck,

    Corresponding author
    1. Molecular Mutagenesis Laboratory, Institut de Recherches Scientifiques sur le Cancer, Centre National de la Recherche Scientifique, BP No.08, Villejuif, 94802, France
      *To whom correspondence should be addressed.
    Search for more papers by this author
  • R. G. Nigro,

    1. Molecular Mutagenesis Laboratory, Institut de Recherches Scientifiques sur le Cancer, Centre National de la Recherche Scientifique, BP No.08, Villejuif, 94802, France
    Search for more papers by this author
  • A. Sarasin,

    1. Institut fur Physiologische Chemie I, University of Dusseldorf, Moorenstr. 5, D-4000 Dusseldorf, W. Germany, department of Biology, Instituto de Biociencias, CP 11461, Universidade de Sao Paulo, Sao Paulo, 05499, SP, Brazil
    Search for more papers by this author
  • H. Sies

    1. Institut fur Physiologische Chemie I, University of Dusseldorf, Moorenstr. 5, D-4000 Dusseldorf, W. Germany, department of Biology, Instituto de Biociencias, CP 11461, Universidade de Sao Paulo, Sao Paulo, 05499, SP, Brazil
    Search for more papers by this author

*To whom correspondence should be addressed.

Abstract

Abstract— We have determined the deleterious effects of singlet oxygen CO,), generated by thermal decomposition of the water-soluble endoperoxide 3,3'-(1,4-naphthylidene)dipropionate (NDPCX), on plasmid DNA. By following the electrophoretic mobility of DNA on agarose gels, we detected single and double strand breaks induced by treatment with NDPO2 The vector employed was a mammalian shuttle vector and the mutagenic consequences of these damages were investigated, using as mutation target the supF suppressor tRNA gene. A high increase of the mutation frequency, over the background, was observed in plasmids transfected in bacteria or after passage through mammalian cells. Trapping agents and quencher effects and other controls confirm the involvement of 'CK in DNA damage and mutagenicity. These findings indicate that 1O2 can induce DNA lesions which are repaired by an error-prone process in prokaryotic and eukaryotic cells.

Ancillary