*Institute de Quimica, CP 20780, USP, Sáo Paulo 05499, SP, Brazil.
SINGLET OXYGEN INDUCED DNA DAMAGE AND MUTAGENICITY IN A SINGLE-STRANDED SV40-BASED SHUTTLE VECTOR
Article first published online: 2 JAN 2008
Photochemistry and Photobiology
Volume 55, Issue 1, pages 39–45, January 1992
How to Cite
Ribeiro, D. T., Madzak, C., Sarasin, A., Mascio, P. D., Sies, H. and Menck, C. F. M. (1992), SINGLET OXYGEN INDUCED DNA DAMAGE AND MUTAGENICITY IN A SINGLE-STRANDED SV40-BASED SHUTTLE VECTOR. Photochemistry and Photobiology, 55: 39–45. doi: 10.1111/j.1751-1097.1992.tb04207.x
- Issue published online: 2 JAN 2008
- Article first published online: 2 JAN 2008
- Received 20 February 1991; accepted 30 May 1991
Abstract— The effects of singlet oxygen (1O2), generated by the thermal decomposition of water soluble NDPO2 (endoperoxide of the disodium 3,3'-(1,4-naphthylidene) dipropionate), on a single-stranded shuttle vector were analysed. 1O2 induces a much higher level of breaks in the phosphodiester backbone of single-stranded than double-stranded DNA. This may be due to a higher accessibility of guanine residue, primarily damaged by 1O2. The damaged vector was transfected into monkey COS7 cells where single-stranded DNA was converted to the double-stranded replicative form DNA. After 3 days, extrachromosomal DNA was extracted and the plasmids rescued in E. coli to study mutagenesis. There is a significant increase in mutation frequency of damaged single-stranded DNA in comparison to untreated DNA. It is concluded that 1O2 induces breaks in the backbone of single-stranded DNA and that the 1O2-damaged molecules are mutated after passage through mammalian cells.