CYCLIC VOLTAMMETRY MEASUREMENTS OF THE PHOTOELECTROGENIC REACTIONS OF THYLAKOID MEMBRANES

Authors

  • Angela Agostiano,

    1. Dipartimento di Chimica, Università di Bari, 4a Traversa Re David 200, 700126 Bari, Italy
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  • D. Christopher Goetze,

    1. Centre de Recherche en Photobiophysique, Université du Québec à Trois Rivières, C.P. 500, Trois Rivières, Québec, Canada G9A 5H7
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    • *University of British Columbia, Department of Botany, Vancouver, Canada TC V6T 2B1.

  • Robert Carpentier

    Corresponding author
    1. Centre de Recherche en Photobiophysique, Université du Québec à Trois Rivières, C.P. 500, Trois Rivières, Québec, Canada G9A 5H7
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†To whom correspondence should be addressed.

Abstract

Abstract— In this paper, the technique of cyclic voltammetry has been used in a photoelectrochemical cell in order to follow the redox species formed in solution by the photo-induced electron transfer between the thylakoids and various acceptors and donors. The photoelectrochemical behavior of artificial electron acceptors (such as 2,5-dichlorobenzoquinone and methylviologen) and donors (such as sym-diphenylcarbazide and durohydroquinone) specific for either Photosystem I or Photosystem II has been investigated. The influence of inhibiting agents (such as 3-(3,4-dichlorophenyl)-1,1-dimethylurea and Tris) on the cell photoresponse has also been characterized, together with the capability of donors to restore the photocurrent. Evidence for H2O2 formation by way of a Mehler-type reaction has been provided and an electrochemical model of its coupled photochemical and electrochemical reactions in solution is reported.

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