LOVASTATIN POTENTIATES THE PHOTOCYTOTOXIC EFFECT OF PHOTOFRIN II DELIVERED TO HT29 HUMAN COLONIC ADENOCARCINOMA CELLS BY LOW DENSITY LIPOPROTEIN

Authors

  • S. Biade,

    1. Laboratoire de Physico-Chimie de l'Adaptation Biologique, INSERM U312, Muséum National d'Histoire Naturelle de Paris, 43 rue Cuvier, 75231 Paris Cedex 05, France
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  • J. C. Mazière,

    Corresponding author
    1. Laboratoire de Physico-Chimie de l'Adaptation Biologique, INSERM U312, Muséum National d'Histoire Naturelle de Paris, 43 rue Cuvier, 75231 Paris Cedex 05, France
    2. Laboratoire de Biochimie, Faculté de Médecine Saint-Antoine, 27 rue Chaligny, 75012 Paris, France
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  • L. Mora,

    1. Laboratoire de Physico-Chimie de l'Adaptation Biologique, INSERM U312, Muséum National d'Histoire Naturelle de Paris, 43 rue Cuvier, 75231 Paris Cedex 05, France
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  • R. Santus,

    1. Laboratoire de Physico-Chimie de l'Adaptation Biologique, INSERM U312, Muséum National d'Histoire Naturelle de Paris, 43 rue Cuvier, 75231 Paris Cedex 05, France
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  • C. Mazière,

    1. Laboratoire de Biochimie, Faculté de Médecine Saint-Antoine, 27 rue Chaligny, 75012 Paris, France
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  • M. Auclair,

    1. Laboratoire de Biochimie, Faculté de Médecine Saint-Antoine, 27 rue Chaligny, 75012 Paris, France
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  • P. Morlière,

    1. Laboratoire de Dermatologie, INSERM U312, Hôpital H. Mondor, 94010 Créteil, France
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  • L. Dubertret

    1. Laboratoire de Dermatologie, INSERM U312, Hôpital H. Mondor, 94010 Créteil, France
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*To whom correspondence should be addressed.

Abstract

A 24 h preculture of HT29–18human colonic adenocarcinoma cells with the sterol synthesis inhibitor lovastatin at concentrations of 0.1-0.5 μM markedly increased the photocytotoxic effect of photofrin II delivered to cells by low density lipoproteins. Under the same conditions, LDL binding and photofrin II (PII) uptake by HT29 cells increased about 1.8-fold and 1.5-fold, respectively. These results suggest that hydroxymethylglutaryl-coenzyme A reductase inhibitors could be useful for potentiating the photodynamic therapy of tumors by PII.

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