A preliminary report of a part of this study was presented at the International Conference on Photodynamic Therapy and Medical Laser Applications held in Milan, Italy, 1992.
PENETRATION POTENCY OF TOPICAL APPLIED δ-AMINOLEVULINIC ACID FOR PHOTODYNAMIC THERAPY OF BASAL CELL CARCINOMA *
Article first published online: 2 JAN 2008
Photochemistry and Photobiology
Volume 59, Issue 1, pages 73–76, January 1994
How to Cite
Szeimies, R.-M., Sassy, T. and Landthaler, M. (1994), PENETRATION POTENCY OF TOPICAL APPLIED δ-AMINOLEVULINIC ACID FOR PHOTODYNAMIC THERAPY OF BASAL CELL CARCINOMA . Photochemistry and Photobiology, 59: 73–76. doi: 10.1111/j.1751-1097.1994.tb05003.x
- Issue published online: 2 JAN 2008
- Article first published online: 2 JAN 2008
- (Received 16 February 1993: accepted 7 July 1993)
Abstract Penetration potency of δ-aminolevulinic acid (ALA) was studied by examining fluorescence of endogenous protoporphyrin IX in different histological types of basal cell carcinoma. Ten basal cell carcinomas were coated with an ointment containing 10% ALA prior to excision; five served as controls. Tumors were excised either 4 h or 12 h after application of ALA using a modified Mohs’micrographic surgical technique. Horizontal sections were cut from deep dermis to tumor surface and examined under a fluorescence microscope. After 4 h of application, only skin appendages demonstrated fluorescence typical of protoporphyrin IX. After 12 h, fluorescence was detectable in tumor cells in deep dermis. The five controls revealed no fluorescence at any site. These results may confirm the high penetration potential of topically applied ALA and its usefulness in photodynamic therapy. For tumors penetrating to deep dermis, an application time of more than 4 h seems necessary, at least when hydrophilic solvents for ALA are used.