INFLUENCE OF THE POOL SIZE OF THE XANTHOPHYLL CYCLE ON THE EFFECTS OF LIGHT STRESS IN A DIATOM: COMPETITION BETWEEN PHOTOPROTECI'ION AND PHOTOINHIBITION

Authors

  • Wafa Arsalane,

    Corresponding author
    1. Photorégulation et dynamique des membranes végétales, URA CNRS 1810, Département de Biologie, Ecole Normale Supérieure, 46 rue d'Ulm, F-75230, Paris Cedex 05, France
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  • Bernard Rousseau,

    1. Photorégulation et dynamique des membranes végétales, URA CNRS 1810, Département de Biologie, Ecole Normale Supérieure, 46 rue d'Ulm, F-75230, Paris Cedex 05, France
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  • Jean-Claude Duval

    1. Photorégulation et dynamique des membranes végétales, URA CNRS 1810, Département de Biologie, Ecole Normale Supérieure, 46 rue d'Ulm, F-75230, Paris Cedex 05, France
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*To whom correspondence should be addressed.

Abstract

Abstract In a study of the relationship between nonphotochemical quenching of fluorescence and the xanthophyll cycle, we show that the diatom Phaeodactylum tricornutum exhibits several interesting characteristics. This xanthophyll cycle consists of only one reversible epoxidating/deepoxidating step (diadinoxanthiddiatoxanthin). Diadinoxan-thin, which increases from 8 to 17 molecules/100 chlorophyll a (Chl a) during the ageing of the culture, was present as two separate pools, with a portion (of about 5 molecules/100 Chl a) which was never deepoxidated. Under a defined irradiance, the time necessary to abolish net photosynthesis increases with the pool size of diadinoxanthin available for deepoxidation. A close correlation is found between nonphotochemical quenching and the relative ratio of diatoxanthin until the photosytem II center is inactivated. The photoprotective effect of diadinoxanthin deepoxidation is limited to the phase during which quenching of the minimum fluorescence (F0) develops.

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