Indications for the Occurrence of Nitric Oxide Synthases in Fungi and Plants and the Involvement in Photoconidiation of Neurospora crassa

Authors


  • *This paper is dedicated to Prof. Dr. Horst Senger on the occasion of his 65th birthday.

†To whom correspondence should be addressed at: Institut für Pflan zenbiochemie, Universität Tübingen, Corrensstr. 41, D-72076 Tübingen, Germany. Fax: 49-7071-640019; e-mail helga.ninnemann@uni-tuebingen.de.

Abstract

Indications for the occurrence of nitric oxide synthases in Dictyostelium, Neurospora, Phycomyces and the leguminous plant Mucuna hassjoo as well as a physiological role of nitric oxide in Neurospora crassa are demonstrated. An exogenous nitric oxide donor, sodium nitroprus-side, inhibited light-stimulated conidiation in N. crassa. Specific inhibitors of nitric oxide synthase, like the arginine derivatives NG-nitro-L-arginine (L-NA) and NG-nitro-L-arginine-methyl ester (L-NAME), enhanced conidiation in darkness and in the light, whereas the stereo-isomer D-NAME was inactive. This communication reports to our knowledge the first time the presence of enzymatic activity of nitric oxide synthase in fungi and a higher plant and an effect of nitric oxide in fungal photophysiology.

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