Delivery of 9-Cis Retinal to Photoreceptors from Bovine Serum Albumin

Authors

  • Zhongzeng Li,

    1. Departments of Pathology & Laboratory Medicine, Medical University of South Carolina, Charleston, SC, USA
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  • Jianling Zhuang,

    1. Departments of Pathology & Laboratory Medicine, Medical University of South Carolina, Charleston, SC, USA
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  • D. Wesley Corson

    Corresponding author
    1. Departments of Pathology & Laboratory Medicine, Medical University of South Carolina, Charleston, SC, USA
    2. Ophthalmology, Medical University of South Carolina, Charleston, SC, USA
      *Division of Research, Department of Pathology and Laboratory Medicine, Medical University of South Carolina, 165 Ashley Avenue, Suite 309, P.O. Box 250908, Charleston, SC 29425, USA. Fax: 843–792–7762; e-mail: corsondw@musc.edu
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*Division of Research, Department of Pathology and Laboratory Medicine, Medical University of South Carolina, 165 Ashley Avenue, Suite 309, P.O. Box 250908, Charleston, SC 29425, USA. Fax: 843–792–7762; e-mail: corsondw@musc.edu

Abstract

Abstract— Retinoids are labile compounds with less than micro-molar solubility in aqueous solutions. They are normally associated with a carrier protein, interphotoreceptor retinoid binding protein, in the interstitial spaces of the retina. In the past, experimental retinoids have been delivered to isolated retinas and photoreceptors using either unprotected ethanolic suspensions or phospholipid vesicles that protect but require burdensome preparation. The objective of these experiments was to characterize the protection of retinoids and their delivery to isolated photoreceptor cells at high concentrations using fatty-acid-free bovine serum albumin (BSA) as a convenient vehicle and protectant. Our results confirm that BSA is a useful protectant and vehicle for delivery of retinoids to isolated retinas and photoreceptors.

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