Ultraviolet-B Radiation Upregulates Expression of Dectin-2 on Epidermal Langerhans Cells by Activating the Gene Promoter

Authors

  • Makoto Bonkobara,

    1. Department of Dermatology, University of Texas Southwestern Medical Center, and the Dermatology Section (Medical Service), Dallas Veterans Affairs Medical Center, Dallas, TX
    2. Department of Veterinary Clinical Pathology, University of Nippon Veterinary and Animal Science, Tokyo, Japan
    Search for more papers by this author
  • Hiroko Yagihara,

    1. Department of Veterinary Clinical Pathology, University of Nippon Veterinary and Animal Science, Tokyo, Japan
    Search for more papers by this author
  • Tatsuo Yudate,

    1. Department of Dermatology, University of Texas Southwestern Medical Center, and the Dermatology Section (Medical Service), Dallas Veterans Affairs Medical Center, Dallas, TX
    2. Department of Dermatology, Kinki University School of Medicine, 377-2, Ohno-Higashi, Osaka-Sayama, Osaka 589-8511, Japan
    Search for more papers by this author
  • Jin-Sung Chung,

    1. Department of Dermatology, University of Texas Southwestern Medical Center, and the Dermatology Section (Medical Service), Dallas Veterans Affairs Medical Center, Dallas, TX
    Search for more papers by this author
  • Tsukimi Washizu,

    1. Department of Veterinary Clinical Pathology, University of Nippon Veterinary and Animal Science, Tokyo, Japan
    Search for more papers by this author
  • Kiyoshi Ariizumi,

    Corresponding author
    1. Department of Dermatology, University of Texas Southwestern Medical Center, and the Dermatology Section (Medical Service), Dallas Veterans Affairs Medical Center, Dallas, TX
      *To whom correspondence should be addressed: Department of Dermatology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9069, USA. Fax: 214-648-0280; e-mail: Kiyoshi.Ariizumi@UTSouthwestern.edu
    Search for more papers by this author
  • Ponciano D. Cruz Jr.

    1. Department of Dermatology, University of Texas Southwestern Medical Center, and the Dermatology Section (Medical Service), Dallas Veterans Affairs Medical Center, Dallas, TX
    Search for more papers by this author

  • Posted on the website on 5 May 2005

*To whom correspondence should be addressed: Department of Dermatology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9069, USA. Fax: 214-648-0280; e-mail: Kiyoshi.Ariizumi@UTSouthwestern.edu

ABSTRACT

Epidermal Langerhans cells (LC) belong to the antigen-presenting cell (APC) family of dendritic cells that can initiate antigen-specific immunogenic or tolerogenic responses. In mice, we have shown ultraviolet-B (UV-B) irradiation to induce long-lasting suppression (tolerance) of contact hyper-sensitivity responses by converting LC from immunogenic to tolerogenic APC. The C-type lectin receptor, dectin-2, expressed preferentially by LC and dendritic cells, has also been shown to be involved in inducing this form of UV-B-induced immunosuppression. These observations led us to question whether UV-B can modulate dectin-2 expression by LC. In ICR mice engineered to express the dectin-2 gene promoter linked to a luciferase reporter gene, we found broadband UV-B treatment in vivo to activate the promoter in LC. In wild-type C3H/HeN mice, we found such treatment in vivo to yield LC with increased dectin-2 expression at both mRNA and protein levels. Broadband UV-B treatment in vitro of bone marrow-derived dendritic cells from these mice also showed upregulated expression of dectin-2 mRNA. These findings lead us to conclude that broadband UV-B upregulates dectin-2 expression in LC by activating the dectin-2 gene promoter. Such amplification suggests that UV-B-induced immunosuppression may be due (at least in part) to augmented dectin-2 expression in LC.

Ancillary