This paper is part of a special issue dedicated to Professor Hasan Mukhtar on the occasion of his 60th birthday.
Ultraviolet-A and -B Differentially Modify the Tyrosine-Kinase Profile of Human Keratinocytes and Induce the Expression of Arg†
Article first published online: 16 NOV 2007
© 2007 The Authors
Photochemistry and Photobiology
Volume 84, Issue 2, pages 261–265, March/April 2008
How to Cite
Klosner, G., Varecka, R., Knobler, R. and Trautinger, F. (2008), Ultraviolet-A and -B Differentially Modify the Tyrosine-Kinase Profile of Human Keratinocytes and Induce the Expression of Arg. Photochemistry and Photobiology, 84: 261–265. doi: 10.1111/j.1751-1097.2007.00235.x
- Issue published online: 16 NOV 2007
- Article first published online: 16 NOV 2007
- Received 29 August 2007, accepted 24 September 2007
To investigate the expression profile of protein tyrosine kinases (PTKs) in normal human epidermal keratinocytes (NHEK) in response to UVA and UVB we employed a reversed transcriptase polymerase chain reaction (PCR) approach using degenerate primers derived from the conserved catalytic domain of PTKs. Quantitative real-time PCR with specific primers was used to confirm the influence of UV on the expression of the identified PTKs. Arg (Abelson-related gene, Abl2) was the PTK with the highest prevalence (30% of all PTKs) and UVA led to a further induction of Arg expression reaching nine-fold mRNA baseline expression at 17 h after irradiation. UVB was followed by an initial downregulation and a subsequent increase in Arg mRNA reaching five-fold baseline levels after 24 h. We conclude that UVA and UVB differentially modify the expression of PTKs in NHEK, and that Arg appears to have a major role in the response of keratinocytes to UV. These results provide a basis for further studies of PTK in UV-induced signaling that regulates protective responses, cell growth and carcinogenesis in the skin.