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The following supplemental materials are available for this article:

Figure  S1   (a) The fluorescence transients in the blue and red end of the probe LDS 750 in BSA at 25°C. We have acquired 15 such transients to construct time-resolved emission spectra (TRES). (b) The TRES of the probe LDS 750 in BSA at 25°C.

Figure  S2   The temporal decay of solvation correlation function, C(t) and fluorescence anisotropy, r(t) (inset) of the probe LDS 750 in BSA at 25°C.

Figure  S3   (a) The temporal decays of the solvation correlation function C(t) of the probe LDS 750 in BSA at 50°C and 75°C. The decay of fluorescence anisotropies, r(t) of the probe LDS 750 in BSA at 50°C (b) and 75°C (c).

Figure  S4   The temporal decays of the solvation correlation function, C(t) of the probe DCM in BSA at different temperature.

Figure  S5   The temporal decays of fluorescence anisotropies r(t) of the probe DCM in BSA at temperatures 25°C (a), 50°C (b) and 75°C (c).

Figure  S6   The temporal decays of the solvation correlation function C(t) of the probe Coumarin 500 in BSA at different temperature.

Figure  S7   The temporal decays of fluorescence anisotropies r(t) of the probe Coumarin 500 in BSA at temperatures 25°C (a), 50°C (b) and 75°C (c).

Table  S1   Solvation correlation function at different temperatures.

Table  S2   Fluorescence anisotropy at different temperatures.

Detailed instrumental techniques, solvation studies of partially folded states of BSA are provided in this section.

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