Respiratory Deficiency Enhances the Sensitivity of the Pathogenic Fungus Candida to Photodynamic Treatment

  1. Yeissa Chabrier-Roselló1,
  2. Thomas H. Foster2,
  3. Soumya Mitra2,
  4. Constantine G. Haidaris1,3,*

Article first published online: 31 JAN 2008

DOI: 10.1111/j.1751-1097.2007.00280.x

Issue

Photochemistry and Photobiology

Photochemistry and Photobiology

Volume 84, Issue 5, pages 1141–1148, September/October 2008

Additional Information

How to Cite

Chabrier-Roselló, Y., Foster, T. H., Mitra, S. and Haidaris, C. G. (2008), Respiratory Deficiency Enhances the Sensitivity of the Pathogenic Fungus Candida to Photodynamic Treatment. Photochemistry and Photobiology, 84: 1141–1148. doi: 10.1111/j.1751-1097.2007.00280.x

Author Information

  1. 1

    Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, NY

  2. 2

    Department of Imaging Sciences, University of Rochester Medical Center, Rochester, NY

  3. 3

    Center for Oral Biology, University of Rochester Medical Center, Rochester, NY

* *Corresponding author email: haid@mail.rochester.edu (Constantine G. Haidaris)

Publication History

  1. Issue published online: 28 AUG 2008
  2. Article first published online: 31 JAN 2008
  3. Received 23 October 2007, accepted 6 December 2007
Corrected by:

Respiratory Deficiency Enhances the Sensitivity Candida to Photodynamic Treatment

Vol. 84, Issue 6, 1605, Article first published online: 3 NOV 2008

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Abstract

Mucosal infections caused by the pathogenic fungus Candida are a significant infectious disease problem and are often difficult to eradicate because of the high frequency of resistance to conventional antifungal agents. Photodynamic treatment (PDT) offers an attractive therapeutic alternative. Previous studies demonstrated that filamentous forms and biofilms of Candida albicans were sensitive to PDT using Photofrin as a photosensitizer. However, early stationary phase yeast forms of C. albicans and Candida glabrata were not adversely affected by treatment. We report that the cationic porphyrin photosensitizer meso-tetra (N-methyl-4-pyridyl) porphine tetra tosylate (TMP-1363) is effective in PDT against yeast forms of C. albicans and C. glabrata. Respiratory-deficient (RD) strains of C. albicans and C. glabrata display a pleiotropic resistance pattern, including resistance to members of the azole family of antifungals, the salivary antimicrobial peptides histatins and other types of toxic stresses. In contrast to this pattern, RD mutants of both C. albicans and C. glabrata were significantly more sensitive to PDT compared to parental strains. These data suggest that intact mitochondrial function may provide a basal level of anti-oxidant defense against PDT-induced phototoxicity in Candida, and reveals pathways of resistance to oxidative stress that can potentially be targeted to increase the efficacy of PDT against this pathogenic fungus.

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