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Increased UV-B Radiation Affects the Viability, Reactive Oxygen Species Accumulation and Antioxidant Enzyme Activities in Maize (Zea mays L.) Pollen

Authors

  • Shiwen Wang,

    1. State Key Lab of Plant Physiology and Biochemistry, Department of Agronomy, Center of Crop Chemical Control, China Agricultural University, Beijing, China
    2. Arid Land Research Center, Tottori University, Hamasaka, Tottori, Japan
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    • These authors contributed equally to this study.

  • Beitao Xie,

    1. State Key Lab of Plant Physiology and Biochemistry, Department of Agronomy, Center of Crop Chemical Control, China Agricultural University, Beijing, China
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    • These authors contributed equally to this study.

  • Lina Yin,

    1. Arid Land Research Center, Tottori University, Hamasaka, Tottori, Japan
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  • Liusheng Duan,

    1. State Key Lab of Plant Physiology and Biochemistry, Department of Agronomy, Center of Crop Chemical Control, China Agricultural University, Beijing, China
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  • Zhaohu Li,

    Corresponding author
    1. State Key Lab of Plant Physiology and Biochemistry, Department of Agronomy, Center of Crop Chemical Control, China Agricultural University, Beijing, China
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  • A. Egrinya Eneji,

    1. State Key Lab of Plant Physiology and Biochemistry, Department of Agronomy, Center of Crop Chemical Control, China Agricultural University, Beijing, China
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  • Wataru Tsuji,

    1. Arid Land Research Center, Tottori University, Hamasaka, Tottori, Japan
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  • Atsushi Tsunekawa

    1. Arid Land Research Center, Tottori University, Hamasaka, Tottori, Japan
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*Corresponding author email: lizhaohu@cau.edu.cn (Zhaohu Li)

Abstract

The increase in UV-B radiation reaching the earth’s surface has prompted extensive studies on the effects of UV-B on plants. However, most of these studies have not addressed the close characteristics related to future survival of plant populations. The purpose of this study was to investigate the effects of UV-B radiation on reactive oxygen species (ROS) accumulation and antioxidant defense system in relation to germination, tube length and viability of maize pollen. Our results indicate that increased UV-B radiation decreased the pollen germination rate and tube length in vitro and also its fertilization ability in the field. Production of O2•− and H2O2 increased by UV-B radiation treatment, and their continuous accumulation resulted in lipid peroxidization. The activities of superoxide dismutase, catalase, peroxidase and DPPH-radical scavenging were decreased by increased UV-B radiation. The increased ROS and lipid peroxidization, and decreased activities of the antioxidants may be attributed to the effects of UV-B radiation on pollen germination, tube growth and fertilization ability.

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