Effect of HeNe Laser Irradiation on Extracellular Matrix Deposition and Expression of Cytokines and Chemokines in Paracoccidioidomycotic Lesions

Authors

  • Patrícia R. A. Nagib,

    1. Institute of Tropical Pathology and Public Health, Federal University of Goiás-UFG, Goiania, Goiás, Brazil
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Jacy Gameiro,

    1. Department of Parasitology, Microbiology and Immunology, Federal University of Juiz de Fora-UFJF, Juiz de Fora, Minas Gerais, Brazil
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Thiago Alves Da Costa,

    1. Department of Anatomy, Cell Biology and Physiology, Institute of Biology, State University of Campinas-UNICAMP, Campinas, São Paulo, Brazil
    Search for more papers by this author
  • Rosária Di Gangi,

    1. Department of Anatomy, Cell Biology and Physiology, Institute of Biology, State University of Campinas-UNICAMP, Campinas, São Paulo, Brazil
    Search for more papers by this author
  • Júlia Da Silva Ribeiro,

    1. Department of Anatomy, Cell Biology and Physiology, Institute of Biology, State University of Campinas-UNICAMP, Campinas, São Paulo, Brazil
    Search for more papers by this author
  • Luciana Campos Paulino,

    1. Natural and Human Sciences, Federal University of ABC-UFABC, São Paulo, Brazil
    Search for more papers by this author
  • Liana Verinaud

    Corresponding author
    1. Department of Anatomy, Cell Biology and Physiology, Institute of Biology, State University of Campinas-UNICAMP, Campinas, São Paulo, Brazil
    Search for more papers by this author

Corresponding author email: verinaud@unicamp.br (Liana Verinaud)

Abstract

Paracoccidioidomycosis is the most prevalent human mycosis in Latin America. Cutaneous lesions are extremely painful and sensitive, and current treatment with antifungal drugs is lengthy and may cause side effects to patients. In this perspective, the helium-neon (HeNe) laser emerges as a novel therapy form due to its ability to heal wounds without changing cell function. In this work, we evaluate the effects of HeNe laser irradiation on extracellular matrix deposition and expression of cytokines and chemokines in cutaneous lesions caused by experimental infection of Balb/c mice. Our results showed decreased levels of pro-inflammatory interleukin (IL)-17 and tumor necrosis factor-α, and of anti-inflammatory IL-10 cytokines in lesions exposed to HeNe laser irradiation. Chemokines CCL3 and CXCL10 showed decreased levels in laser-treated lesions, but no significant difference was observed in relation to CCL5 expression. We also detected decreased density of fibronectin and laminin in HeNe laser-treated lesions. Data presented herein support the validity of our previous results suggesting positive effects of HeNe laser in accelerating wound healing in this experimental model. We believe that HeNe laser is a new nonharmful strategy that may be used as adjuvant and/or alternative therapy for improving treatment of paracoccidioidomycotic lesions.

Ancillary