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Nitric Oxide-dependent Pigment Migration Induced by Ultraviolet Radiation in Retinal Pigment Cells of the Crab Neohelice granulata

Authors

  • Daza De Moraes Vaz Batista Filgueira,

    1. Programa de Pós-graduação em Ciências Fisiológicas, Fisiologia Animal Comparada, FURG, Instituto de Ciências Biológicas, Rio Grande, Brazil
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  • Laís Pereira Guterres,

    1. Instituto de Ciências Biológicas, Universidade Federal do Rio Grande – FURG, Rio Grande, Brazil
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  • Ana Paula De Souza Votto,

    1. Programa de Pós-graduação em Ciências Fisiológicas, Fisiologia Animal Comparada, FURG, Instituto de Ciências Biológicas, Rio Grande, Brazil
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  • Marcelo Alves Vargas,

    1. Programa de Pós-graduação em Ciências Morfológicas, Universidade Federal do Rio de Janeiro – UFRJ, Rio de Janeiro, Brazil
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  • Robert Tew Boyle,

    1. Instituto de Ciências Biológicas, Universidade Federal do Rio Grande – FURG, Rio Grande, Brazil
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  • Gilma Santos Trindade,

    1. Instituto de Ciências Biológicas, Universidade Federal do Rio Grande – FURG, Rio Grande, Brazil
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  • Luiz Eduardo Maia Nery

    Corresponding author
    1. Instituto de Ciências Biológicas, Universidade Federal do Rio Grande – FURG, Rio Grande, Brazil
      Corresponding author email: nery@octopus.furg.br (Luiz Eduardo Maia Nery)
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Corresponding author email: nery@octopus.furg.br (Luiz Eduardo Maia Nery)

Abstract

The purpose of this study was to verify the occurrence of pigment dispersion in retinal pigment cells exposed to UVA and UVB radiation, and to investigate the possible participation of a nitric oxide (NO) pathway. Retinal pigment cells from Neohelice granulata were obtained by cellular dissociation. Cells were analyzed for 30 min in the dark (control) and then exposed to 1.1 and 3.3 J cm−2 UVA, 0.07 and 0.9 J cm−2 UVB, 20 nmβ-PDH (pigment dispersing hormone) or 10 μm SIN-1 (NO donor). Histological analyses were performed to verify the UV effect in vivo. Cultured cells were exposed to 250 μm L-NAME (NO synthase blocker) and afterwards were treated with UVA, UVB or β-PDH. The retinal cells in culture displayed significant pigment dispersion in response to UVA, UVB and β-PDH. The same responses to UVA and UVB were observed in vivo. SIN-1 did not induce pigment dispersion in the cell cultures. l-NAME significantly decreased the pigment dispersion induced by UVA and UVB but not by β-PDH. All retinal cells showed an immunopositive reaction against neuronal nitric oxide synthases. Therefore, UVA and UVB radiation are capable of inducing pigment dispersion in retinal pigment cells of Neohelice granulata and this dispersion may be nitric oxide synthase dependent.

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