These two authors contributed equally to this work.
Short Exposure to the DNA Intercalator DRAQ5 Dislocates the Transcription Machinery and Induces Cell Death
Article first published online: 10 DEC 2010
© 2010 The Authors. Photochemistry and Photobiology © 2010 The American Society of Photobiology
Photochemistry and Photobiology
Volume 87, Issue 1, pages 256–261, January/February 2011
How to Cite
Richard, E., Causse, S., Spriet, C., Fourré, N., Trinel, D., Darzacq, X., Vandenbunder, B. and Heliot, L. (2011), Short Exposure to the DNA Intercalator DRAQ5 Dislocates the Transcription Machinery and Induces Cell Death. Photochemistry and Photobiology, 87: 256–261. doi: 10.1111/j.1751-1097.2010.00852.x
- Issue published online: 22 DEC 2010
- Article first published online: 10 DEC 2010
- Accepted manuscript online: 4 NOV 2010 12:40PM EST
- Received 8 April 2010, accepted 22 October 2010
The fluorescent probeDRAQ5 which rapidly permeates cells and binds to DNA is potentially useful for functional studies of molecular dynamics and interactions in living nuclei. Within minutes after the incubation of human osteosarcoma U2OS cells with 5 μm DRAQ5, the distributions of RNA polymerase II and some of its associated regulatory proteins HEXIM and cyclin T1 in the nucleus are severely impaired, and transcription is inhibited. Furthermore, 30 min exposure to DRAQ5 induces death of U2OS cells 24 h later. Incubation with Hoechst 33342 under similar conditions does not induce these effects. These results emphasize the importance of carefully examining the functional consequences of labeling DNA with intercalating fluorescent dyes before use.