These authors contributed equally to this work.
Detection of Nucleic Acid Lesions During Photochemical Inactivation of RNA Viruses by Treatment with Methylene Blue and Light Using Real-time PCR
Article first published online: 11 JAN 2011
© 2011 Shanghai Blood Center, China. Photochemistry and Photobiology © 2011 The American Society of Photobiology
Photochemistry and Photobiology
Volume 87, Issue 2, pages 365–369, March/April 2011
How to Cite
Zhang, B., Zheng, L., Huang, Y., Mo, Q., Wang, X. and Qian, K. (2011), Detection of Nucleic Acid Lesions During Photochemical Inactivation of RNA Viruses by Treatment with Methylene Blue and Light Using Real-time PCR. Photochemistry and Photobiology, 87: 365–369. doi: 10.1111/j.1751-1097.2010.00870.x
- Issue published online: 1 MAR 2011
- Article first published online: 11 JAN 2011
- Accepted manuscript online: 8 DEC 2010 05:18AM EST
- Received 21 July 2010, accepted 22 November 2010
The mechanism of bacteriophage photoinactivation by methylene blue and light (MB+L) involves genomic RNA damage. In this study, two RNA viruses, Sindbis virus (SINV) and hepatitis C virus were treated by MB+L and their nucleic acids were amplified to show that RNA lesions occurred during inactivation. During MB+L inactivation, the viral load of both viruses was significantly reduced as MB+L exposure increased. The nucleic acid amplification of treated viral RNA was inhibited in a time-dependent manner and the percentage inhibition of amplification reached about 99% after 30 min of treatment. Furthermore, as compared to SINV viral infectivity detected by quantification of the 50% tissue culture infective dose (TCID50), the inhibition of SINV RNA amplification strongly correlated with a decrease in in vitro infectivity (R2 > 0.94), suggesting that RNA serves as the main target during MB+L inactivation.