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In Vivo Comparison of the Optical Clearing Efficacy of Optical Clearing Agents in Human Skin by Quantifying Permeability Using Optical Coherence Tomography

Authors

  • Xiao Guo,

    1. MOE Key laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, Guangzhou, Guangdong Province, China
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  • Zhouyi Guo,

    1. MOE Key laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, Guangzhou, Guangdong Province, China
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  • Huajiang Wei,

    Corresponding author
    1. MOE Key laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, Guangzhou, Guangdong Province, China
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  • Hongqin Yang,

    1. Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education of China, Fujian Normal University, Fuzhou, Fujian, China
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  • Yonghong He,

    1. Graduate School at Shenzhen, Tsinghua University, Shenzhen, Guangdong, China
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  • Shusen Xie,

    1. Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education of China, Fujian Normal University, Fuzhou, Fujian, China
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  • Guoyong Wu,

    1. Department of Surgery, the First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, Guangdong Province, China
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  • Xiaoyuan Deng,

    1. MOE Key laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, Guangzhou, Guangdong Province, China
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  • Qingliang Zhao,

    1. MOE Key laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, Guangzhou, Guangdong Province, China
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  • Lanquan Li

    1. MOE Key laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, Guangzhou, Guangdong Province, China
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Corresponding author email: weihj@scnu.edu.cn (Huajiang Wei)

Abstract

The objective of this work is to quantify and compare the optical clearing efficacy of glucose, propylene glycol, glycerol solutions through the human skin tissue in vivo by calculating permeability coefficient of three solutions. Currently, the permeability coefficient of agent in tissues was extracted from optical coherence tomography (OCT) amplitude data mainly through the OCT signal slope and the OCT amplitude methods. In this study, we report the OCT attenuation coefficient method which is a relatively novel and rarely reported methodology to measure the permeability coefficient during the optical skin clearing procedure. The permeability coefficients for 40% propylene glycol, glucose and glycerol were (2.74 ± 0.05) × 10−6 cm s−1, (1.78 ± 0.04) × 10−6 cm s−1 and (1.67 ± 0.04) × 10−6 cm s−1, respectively. It could be clearly seen that the permeability coefficient of the 40% propylene glycol solution is higher than that of 40% glucose solution, and the permeability coefficient of the 40% glucose solution is higher than that of the 40% glycerol solution. These indicate 40% propylene glycol solution is more effective than others in the human skin in vivo. We then compare and prove consistency of optical clearing efficacy figured out by three different methods.

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