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Photodynamic Effects of Pterin on HeLa Cells

Authors

  • M. Paula Denofrio,

    1. Departamento de Química, Facultad de Ciencias Exactas, Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Universidad Nacional de La Plata, CCT La Plata-CONICET, La Plata, Argentina
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  • Carolina Lorente,

    1. Departamento de Química, Facultad de Ciencias Exactas, Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Universidad Nacional de La Plata, CCT La Plata-CONICET, La Plata, Argentina
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  • Thomas Breitenbach,

    1. Department of Chemistry, Center for Oxygen Microscopy and Imaging, University of Aarhus, Århus, Denmark
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  • Sonja Hatz,

    1. Department of Chemistry, Center for Oxygen Microscopy and Imaging, University of Aarhus, Århus, Denmark
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  • Franco M. Cabrerizo,

    Corresponding author
    1. Instituto de Investigaciones Biotecnológicas, Instituto Tecnológico de Chascomús (IIB-INTECH), Universidad Nacional de San Martín, CONICET, Camino Circunv., Chascomús, Argentina
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  • Andrés H. Thomas,

    Corresponding author
    1. Departamento de Química, Facultad de Ciencias Exactas, Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Universidad Nacional de La Plata, CCT La Plata-CONICET, La Plata, Argentina
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  • Peter R. Ogilby

    1. Department of Chemistry, Center for Oxygen Microscopy and Imaging, University of Aarhus, Århus, Denmark
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Corresponding author email: fcabrerizo@intech.gov.ar (Franco M. Cabrerizo), athomas@inifta.unlp.edu.ar (Andrés H. Thomas), progilby@chem.au.dk (Peter R. Ogilby)

Abstract

Pterins, heterocyclic compounds widespread in biological systems, participate in relevant biological processes and are able to act as photosensitizers. In the present study, we ascertained that 2-aminopteridin-4(3H)-one, abbreviated as Ptr, is readily incorporated into and/or onto cervical cancer cells (HeLa) and that these cells die upon UV-A irradiation of Ptr. Cell death was assessed using two tests: (1) the Rhodamine 123 fluorescence assay for mitochondrial viability and (2) the Trypan Blue assay for membrane integrity. The data suggest that, for Ptr-dependent photoinitiated cell death, events related to mitochondrial failure precede those associated with the failure of the cell membrane.

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