Light-activated Antibacterial Surfaces Comprise Photosensitizers

Authors

  • Rivka Cahan,

    Corresponding author
    1. Department of Chemical Engineering and Biotechnology, Ariel University Center of Samaria, Ariel, Israel
      Corresponding author email: rivkac@ariel.ac.il (Rivka Cahan)
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  • Ronen Schwartz,

    1. Department of Chemical Engineering and Biotechnology, Ariel University Center of Samaria, Ariel, Israel
    2. The Mina & Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel
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  • Yakov Langzam,

    1. The Mina & Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel
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  • Yeshayahu Nitzan

    1. The Mina & Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel
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Corresponding author email: rivkac@ariel.ac.il (Rivka Cahan)

ABSTRACT

Antibacterial surfaces were prepared using a base polyethylene sheet topped with a layer containing a mixed powder of poly (vinylidene fluoride) and photosensitizers (PSs). A crimpled stamp was placed on the mixed powder, and then it was passed through a heating and pressing device. The three chosen PSs were rose bengal, toluidine blue O and methylene blue. Scanning electron microscope analysis showed that the PS surface texture was coarse and highly developed. Measurement of the apparent contact angles of the droplets deposited on the PS surfaces using goniometry showed that all three surfaces were hydrophobic. Photodynamic analysis of the surfaces into which the PSs were incorporated indicated significant reactive oxygen species formation after illumination with light fluency rate of 1.46 mW cm−2 for 30 min. Photodynamic inactivation assays performed in nutrient broth demonstrated more than 4 log reduction of the attached Escherichia coli after illumination (1.46 mW cm−2) for 24 h when the inoculum was 103 CFU mL−1. However, more than 4 log reduction of Staphylococcus aureus occurred even when the cultures were illuminated for only 6 h. Our results provide an inexpensive, simple, state-of-the-art method for preparing antibacterial surfaces that may help prevent infections in hospital surroundings and in some medical devices.

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