Figure S1. Human primary keratinocytes were pulse-labeled with 20 mM 5’-bromo-2’deoxyuridine (BrdU) for 30 min. The Cells were fixed with 4% formaldehyde at room temperature for 15 min and subsequently permeabilized with ice-cold 0.5% TritonX-100 in 10 mM PBS (pH 7.4) on ice for 5 min. After denaturing cellular DNA with 2 M HCl at room temperature for 30 min, the cells were stained with anti-BrdU antibody conjugated with Alexa Fluor-594 (Invitrogen), and counterstained with 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) (Invitrogen). To obtain fluorescence images, BIOREVO BZ-9000 fluorescence microscope (Keyence) was used.

PHP_1073_sm_FigS1.pdf3985KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.