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Figure S1. Human primary keratinocytes were pulse-labeled with 20 mM 5’-bromo-2’deoxyuridine (BrdU) for 30 min. The Cells were fixed with 4% formaldehyde at room temperature for 15 min and subsequently permeabilized with ice-cold 0.5% TritonX-100 in 10 mM PBS (pH 7.4) on ice for 5 min. After denaturing cellular DNA with 2 M HCl at room temperature for 30 min, the cells were stained with anti-BrdU antibody conjugated with Alexa Fluor-594 (Invitrogen), and counterstained with 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) (Invitrogen). To obtain fluorescence images, BIOREVO BZ-9000 fluorescence microscope (Keyence) was used.

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