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Tualang Honey Protects Keratinocytes from Ultraviolet Radiation-Induced Inflammation and DNA Damage

Authors

  • Israr Ahmad,

    1. Department of Dermatology and Skin Diseases Research Center, Veterans Affairs Medical Center, Birmingham, AL
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  • Hugo Jimenez,

    1. Department of Dermatology and Skin Diseases Research Center, Veterans Affairs Medical Center, Birmingham, AL
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  • Nik Soriani Yaacob,

    1. School of Medical Sciences, Universiti Sains Malaysia, Malaysia
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  • Nabiha Yusuf

    Corresponding author
    1. Department of Dermatology and Skin Diseases Research Center, Veterans Affairs Medical Center, Birmingham, AL
    2. Comprehensive Cancer Center, University of Alabama at Birmingham, AL
    3. School of Medical Sciences, Universiti Sains Malaysia, Malaysia
      Corresponding author email: nabiha@uab.edu (Nabiha Yusuf)
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  • This paper is part of the Special Issue in Commemoration of the 70th Birthday of Dr. David R. Bickers.

Corresponding author email: nabiha@uab.edu (Nabiha Yusuf)

Abstract

Malaysian tualang honey possesses strong antioxidant and anti-inflammatory properties. Here, we evaluated the effect of tualang honey on early biomarkers of photocarcinogenesis employing PAM212 mouse keratinocyte cell line. Keratinocytes were treated with tualang honey (1.0%, v/v) before a single UVB (150 mJ cm−2) irradiation. We found that the treatment of tualang honey inhibited UVB-induced DNA damage, and enhanced repair of UVB-mediated formation of cyclobutane pyrimidine dimers and 8-oxo-7,8-dihydro-2′-deoxyguanosine. Treatment of tualang honey inhibited UVB-induced nuclear translocation of NF-κB and degradation of IκBα in murine keratinocyte cell line. The treatment of tualang honey also inhibited UVB-induced inflammatory cytokines and inducible nitric oxide synthase protein expression. Furthermore, the treatment of tualang honey inhibited UVB-induced COX-2 expression and PGE2 production. Taken together, we provide evidence that the treatment of tualang honey to keratinocytes affords substantial protection from the adverse effects of UVB radiation via modulation in early biomarkers of photocarcinogenesis and provide suggestion for its photochemopreventive potential.

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