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Effect of Low-Level Laser Therapy (660 nm) on Acute Inflammation Induced by Tenotomy of Achilles Tendon in Rats

Authors

  • Erica Martinho Salvador Laraia,

    1. Post Graduate Program for Health and Development of the Central-West Region, Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil
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  • Iandara Schettert Silva,

    1. Post Graduate Program for Health and Development of the Central-West Region, Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil
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  • Daniel Martins Pereira,

    1. Post Graduate Program for Health and Development of the Central-West Region, Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil
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  • Filipe Abdalla dos Reis,

    1. Post Graduate Program for Health and Development of the Central-West Region, Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil
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  • Regiane Albertini,

    1. Post Graduate Program in Rehabilitation Sciences, Nove de Julho University (UNINOVE), São Paulo, SP, Brazil
    2. Post Graduate Program in Biophotonics, Nove de Julho University (UNINOVE), São Paulo, SP, Brazil
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  • Patrícia de Almeida,

    1. Post Graduate Program in Rehabilitation Sciences, Nove de Julho University (UNINOVE), São Paulo, SP, Brazil
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  • Ernesto Cesar Pinto Leal Junior,

    1. Post Graduate Program in Rehabilitation Sciences, Nove de Julho University (UNINOVE), São Paulo, SP, Brazil
    2. Post Graduate Program in Biophotonics, Nove de Julho University (UNINOVE), São Paulo, SP, Brazil
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  • Paulo de Tarso Camillo de Carvalho

    Corresponding author
    1. Post Graduate Program in Rehabilitation Sciences, Nove de Julho University (UNINOVE), São Paulo, SP, Brazil
    2. Post Graduate Program in Biophotonics, Nove de Julho University (UNINOVE), São Paulo, SP, Brazil
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Corresponding author email: ptpaulo@terra.com.br (Paulo de Tarso Camillo de Carvalho)

Abstract

In this study, we aimed to analyze the effects of low-level laser therapy (LLLT; 660 nm) on levels of protein expression of inflammatory mediators after cutting Achilles tendon of rats. Thirty Wistar male rats underwent partial incisions of the left Achilles tendon, and were divided into three groups of 10 animals according to the time of euthanasia after injury: 6, 24 and 72 h. Each group was then divided into control group and LLLT group (treated with 100 mW, 3.57 W cm−2, 0.028 cm2, 214 J cm−2, 6 J, 60 s, single point). In LLLT group, animals were treated once time per day until the time of euthanasia established for each group. The group treated with LLLT showed a significant reduction of IL-1β compared with control groups at three time points (6 h: = 0.0401; 24 h: = 0.0015; 72 h: = 0.0463). The analysis of IL-6 showed significant reduction only in the LLLT group at 72 h compared with control group (= 0.0179), whereas IL-10 showed a significant increase in the treated group compared with control group at three experimental times (6 h: = 0.0007; 24 h: = 0.0256; 72 h: < 0.0001). We conclude that LLLT is an important modulator of inflammatory cytokines release after injury in Achilles tendon.

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