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php1217-sup-0001-FigureS1.tifimage/tif647KFigure S1. Singlet oxygen quantum yields of hypericin (in red) and Foslipos® (in blue) at excitation wavelengths of 415 nm for Foslipos® (with increasing hypericin concentration) and 590 nm for hypericin (with increasing Foslipos® concentration). Rose Bengal was used as a reference solution.
php1217-sup-0002-FigureS2.jpgimage/jpg488KFigure S2. The figure is exemplarily illustrating different cell stainings and morphological changes post PDT mediated by hypericin treatment in head and neck squamous cell carcinoma cells. (A) DIC and confocal pictures (CM-H2DCFDA staining) of UMB-SCC 969 cell line indicating different stages of cell death (A1: control cells, A2: cells 2 h after illumination and A3: cells 3 h after illumination). (B) confocal scanning pictures illustrating (on top) the cells stained with FLICA caspases assay (DAPI in blue, hypericin in red and caspase stain in green) immediately after illumination and 2 h later (UMB-SCC 969), (in the middle) annexin V death assay (DAPI in blue, hypericin in red and annexin V stain in green) after 1 and 3 h post illumination (UMB-SCC 745) and (on the bottom) cytochrome C staining (DAPI in blue and cytochrome C in yellow) directly after illumination and 3 h later (UMB-SCC 969). Scale bars = 30 μm.
php1217-sup-0003-FigureS3.jpgimage/jpg570KFigure S3. The figure is exemplarily illustrating different cell stainings and morphological changes post PDT mediated by Foslipos® treatment in head and neck squamous cell carcinoma cells. (A) DIC and confocal pictures (CM-H2DCFDA staining) of UMB-SCC 969 cell line indicating different stages of cell death (A1: control cells, A2: cells 2 h after illumination and A3: cells 4 h after illumination). (B) confocal scanning pictures illustrating (on top) the cells stained with FLICA caspases assay (DAPI in blue, hypericin in red and caspase stain in green) immediately after illumination and 3 h later (UMB-SCC 969), (in the middle) annexin V death assay (DAPI in blue, hypericin in red and annexin V stain in green) after 1 and 3 h post illumination (UMB-SCC 745)and (on the bottom) cytochrome C staining (DAPI in blue and cytochrome C in yellow) directly after illumination and 3 h later (UMB-SCC 969). Scale bars = 30 μm.
php1217-sup-0004-FigureS4.jpgimage/jpg541KFigure S4. The figure is exemplarily illustrating different cell stainings and morphological changes post PDT mediated by mixture of hypericin and Foslipos® treatment. (A) DIC and confocal pictures (CM-H2DCFDA staining) of UMB-SCC 969 cell line indicating different stages of cell death (A1: control cells, A2: cells 2 h after illumination and A3: cells 3 h after illumination). (B) confocal scanning pictures illustrating (on top) the cells stained with FLICA caspases assay (DAPI in blue, hypericin in red and caspase stain in green) immediately after illumination and 3 h later (UMB-SCC 745), (in the middle) annexin V death assay (DAPI in blue, hypericin in red and annexin V stain in green) after 1 and 3 h post illumination (UMB-SCC 745) and (on the bottom) cytochrome C staining (DAPI in blue and cytochrome C in yellow) directly after illumination and 3 h later (UMB-SCC 969). Scale bars = 30 μm.
php1217-sup-0005-movieS1.avivideo/avi5430KMovie S1. The live cell monitoring after the hypericin treatment (2.5 μg mL−1, 5 h of incubation, 1 min illumination with 6000 Lx; 250 μW; 32 mW cm−2). Pictures were made with wide field microscope Leica LX every 10 min during 18 h.
php1217-sup-0006-movieS2.avivideo/avi5112KMovie S2. The live cell monitoring after the Foslipos treatment (2.5 μg mL−1, 5 h of incubation, 1 min illumination with 6000 Lx; 250 μW; 32 mW cm−2). Pictures were made with wide field microscope Leica LX every 10 min during 18 h.
php1217-sup-0007-movieS3.avivideo/avi5450KMovie S3. The live cell monitoring after the photosensitizer mixture treatment (1.25 μg mL−1 each photosensitizer, 5 h of incubation, 1 min illumination with 6000 Lx; 250 μW; 32 mW cm−2). Pictures were made with wide field microscope Leica LX every 10 min during 18 h.

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