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Modulation of Lipopolysaccharide-Induced NF-κB Signaling Pathway by 635 nm Irradiation via Heat Shock Protein 27 in Human Gingival Fibroblast Cells

Authors

  • WonBong Lim,

    1. Department of Oral Pathology, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea
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    • The first two authors contributed equally to this work.

  • JiSun Kim,

    1. Department of Oral Pathology, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea
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    • The first two authors contributed equally to this work.

  • SangWoo Kim,

    1. Department of Oral Pathology, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea
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  • Sandeep Karna,

    1. Department of Oral Pathology, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea
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  • JaeWoong Won,

    1. Department of Oral Pathology, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea
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  • Sang Mi Jeon,

    1. Department of Oral Pathology, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea
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  • Seo Yeon Kim,

    1. Department of Oral Pathology, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea
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  • YooDuk Choi,

    1. Department of Pathology, Faculty of Medicine, Chonnam National University, Gwangju, Korea
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  • HongRan Choi,

    1. Department of Oral Pathology, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea
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  • OkJoon Kim

    Corresponding author
    • Department of Oral Pathology, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea
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Corresponding author email: js3894@chonnam.ac.kr (OkJoon Kim)

Abstract

Heat shock protein-27 (HSP27) is a member of the small HSP family which has been linked to the nuclear factor-kappa B (NF-κB) signaling pathway regulating inflammatory responses. Clinical reports have suggested that low-level light therapy/laser irradiation (LLLT) could be an effective alternative treatment to relieve inflammation during bacterial infection associated with periodontal disease. However, it remains unclear how light irradiation can modulate the NF-κB signaling pathway. We examined whether or not 635 nm irradiation could lead to a modulation of the NF-kB signaling pathway in HSP27-silenced cells and analyzed the functional cross-talk between these factors in NF-κB activation. The results showed that 635 nm irradiation led to a decrease in the HSP27 phosphorylation, reactive oxygen species (ROS) generation, I-κB kinase (IKK)/inhibitor of κB (IκB)/NF-κB phosphorylation, NF-κB p65 translocation and a subsequent decrease in the COX-1/2 expression and prostaglandin (PGE2) release in lipopolysaccharide(LPS)-induced human gingival fibroblast cells (hGFs). However, in HSP27-silenced hGFs, no obvious changes were observed in ROS generation, IKK/IκB/NF-κB phosphorylation, NF-κB p65 translocation, nor in COX-1/2 expression, or PGE2 release. This could be a mechanism by which 635 nm irradiation modulates LPS-induced NF-κB signaling pathway via HSP27 in inflammation. Thus, HSP27 may play a role in regulating the anti-inflammatory response of LLLT.

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