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Ethanol Extract of Peanut Sprout Induces Nrf2 Activation and Expression of Antioxidant and Detoxifying Enzymes in Human Dermal Fibroblasts: Implication for its Protection Against UVB-irradiated Oxidative Stress

Authors

  • Jee-Young Choi,

    1. Department of Dermatology of Chonnam National University Medical School, Chonnam National University, Gwangju, Korea
    2. Chonnam National University Hospital Research Institute of Clinical Medicine, Chonnam National University, Gwangju, Korea
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  • Da-In Choi,

    1. Department of Dermatology of Chonnam National University Medical School, Chonnam National University, Gwangju, Korea
    2. Chonnam National University Hospital Research Institute of Clinical Medicine, Chonnam National University, Gwangju, Korea
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  • Jee-Bum Lee,

    1. Department of Dermatology of Chonnam National University Medical School, Chonnam National University, Gwangju, Korea
    2. Chonnam National University Hospital Research Institute of Clinical Medicine, Chonnam National University, Gwangju, Korea
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  • Suk-Jung Yun,

    1. Department of Dermatology of Chonnam National University Medical School, Chonnam National University, Gwangju, Korea
    2. Chonnam National University Hospital Research Institute of Clinical Medicine, Chonnam National University, Gwangju, Korea
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  • Dong-Ho Lee,

    1. Chonnam National University Hospital Research Institute of Clinical Medicine, Chonnam National University, Gwangju, Korea
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  • Jong-Bang Eun,

    1. Department of Food Science and Technology and Functional Food Research Center, Chonnam National University, Gwangju, Korea
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  • Seung-Chul Lee

    Corresponding author
    1. Chonnam National University Hospital Research Institute of Clinical Medicine, Chonnam National University, Gwangju, Korea
    • Department of Dermatology of Chonnam National University Medical School, Chonnam National University, Gwangju, Korea
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Corresponding author email: schul@jnu.ac.kr (Seung-Chul Lee)

Abstract

A peanut sprout is known to contain a significant level of resveratrol, which was reported to have beneficial effects in our body due to its antioxidant activities. The purpose of this study was to evaluate the cytoprotective activity of ethanol extract of peanut sprout (EPS) from ultraviolet B (UVB)-induced oxidative stress in human dermal fibroblasts (HDF). EPS was revealed to contain 54.2 μg g−1 of trans-resveratrol. The DCF-DA-positive reactive oxygen species level was increased by 50 mJ cm−2 of UVB irradiation (2150 ± 450% of nonirradiated control), which was markedly suppressed by EPS treatment (180 ± 42% of control). Annexin V-positive apoptotic cell death induced by UVB irradiation (16.4 ± 4.5%) was also significantly inhibited by EPS treatment (6.7 ± 2.5%). EPS induced up-regulation and nuclear translocation of Nrf2, a transcription factor for antioxidant and detoxifying enzymes, in HDF as a dose-dependent manner. UVB irradiation up-regulated Nrf2-dependent enzymes of heme oxygenase-1, NAD(P)H:quinine oxidoreductase-1 and glutathione-S-transferase pi, and they were further stimulated by EPS treatment. Taken together, EPS is an efficient cytoprotective agent against UVB-induced oxidative stress by activation of Nrf2 and upregulation of Nrf2-relating antioxidant and detoxifying enzymes in HDF.

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