Conjugation of methotrexate to immunoglobulins kills macrophages by Fc receptor mediated uptake?
Article first published online: 30 JUL 2007
© 2007 The Authors. Journal compilation © 2007 Blackwell Publishing Ltd
International Journal of Laboratory Hematology
Volume 30, Issue 3, pages 185–190, June 2008
How to Cite
WANG, X., YAO, C. and JIANG, Z. (2008), Conjugation of methotrexate to immunoglobulins kills macrophages by Fc receptor mediated uptake?. International Journal of Laboratory Hematology, 30: 185–190. doi: 10.1111/j.1751-553X.2007.00936.x
- Issue published online: 28 JUN 2008
- Article first published online: 30 JUL 2007
- Received 9 December 2006; accepted for publication 29 January 2007
- Idiopathic thrombocytopenic purpura;
- intravenous immunoglobulin;
- Fc receptors;
The aim of this study was to conjugate methotrexate (MTX) with intravenous immunoglobulin (IVIG) and investigate whether the conjugate produce selective cytotoxicity on macrophages to provide a new strategy for the management of idiopathic thrombocytopenic purpura. MTX was bound to IVIG via human serum albumin as an intermediary. The binding activity of the Fc fragment of the conjugate was assayed by flow cytometry. The selective cytotoxicity of the conjugate was determined by trypan blue exclusion. After conjugating, the binding activity of the conjugate to Fc receptors did not diminish when compared with IVIG. In vitro, the conjugate showed significantly higher cytotoxicity to macrophages than Hela cells. The conjugate of IVIG and MTX showed potent and selective cytotoxicity to macrophages in vitro.