Comparison of array comparative genomic hybridization (aCGH) to FISH and cytogenetics in prognostic evaluation of chronic lymphocytic leukemia
Article first published online: 9 DEC 2010
© 2010 Blackwell Publishing Ltd
International Journal of Laboratory Hematology
Volume 33, Issue 3, pages 238–244, June 2011
How to Cite
O’MALLEY, D. P., GIUDICE, C., CHANG, A. S., CHANG, D., BARRY, T. S., HIBBARD, M. K., CHEN, R. and CHEN, S.-T. (2011), Comparison of array comparative genomic hybridization (aCGH) to FISH and cytogenetics in prognostic evaluation of chronic lymphocytic leukemia. International Journal of Laboratory Hematology, 33: 238–244. doi: 10.1111/j.1751-553X.2010.01284.x
- Issue published online: 15 APR 2011
- Article first published online: 9 DEC 2010
- Received 23 July 2010; accepted for publication 27 September 2010
Introduction: High-resolution array comparative genomic hybridization (aCGH) is a method of evaluating chromosomal alterations over the entire genome. We compared aCGH with routine cytogenetics and FISH in detecting genetic alterations in chronic lymphocytic leukemia (CLL).
Methods: Array comparative genomic hybridization testing was performed on 55 cases of CLL in addition to a standard panel of FISH probes (ATM on 11q22, trisomy 12, 13q14, p53 on 17p13). The frequency of detecting abnormalities was compared, and discordant results between methodologies were compared.
Results: Fifty-five CLL cases [male to female ratio of 2.2:1 and a mean age of 71 (52–90)] were analyzed by both aCGH and FISH. This group of CLL cases showed genetic abnormalities by FISH (60%; 27/45). In contrast to FISH, aCGH detected genetic abnormalities in 82% (45/55) of CLL cases; aCGH identified genetic abnormalities not detected by FISH studies in 16% (7/45) of cases, whereas FISH identified abnormalities not detected by aCGH in only 7% (3/45) of cases. Rare recurring genetic alterations were detected by aCGH including losses in 6q, 8p, 10q, 14q32, and 18q and gains in 10q.
Discussion: Our findings suggest aCGH is an effective technique for evaluating recurring genetic abnormalities in CLL and improves on standard FISH in detecting genetic abnormalities in CLL.