• Open Access

Cyclopropane fatty acids are involved in organic solvent tolerance but not in acid stress resistance in Pseudomonas putida DOT-T1E

Authors

  • Cecilia-Vanesa Pini,

    1. Environmental Protection Department, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, C/Profesor Albareda 1, 18008-Granada, Spain.
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  • Patricia Bernal,

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    • Present address: Microbiology Group, Department of Pharmaceutics, School of Pharmacy, University of London, 29/39 Brunswick Square, London WC1N 1AX, UK.

  • Patricia Godoy,

    1. Environmental Protection Department, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, C/Profesor Albareda 1, 18008-Granada, Spain.
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  • Juan-Luis Ramos,

    1. Environmental Protection Department, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, C/Profesor Albareda 1, 18008-Granada, Spain.
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  • Ana Segura

    Corresponding authorSearch for more papers by this author

*E-mail ana.segura@eez.csic.es; Tel. (+ 34) 958 181600; Fax (+ 34) 958 129600.

Summary

Bacterial membranes constitute the first physical barrier against different environmental stresses. Pseudomonas putida DOT-T1E accumulates cyclopropane fatty acids (CFAs) in the stationary phase of growth. In this strain the cfaB gene encodes the main cyclopropane synthase responsible of the synthesis of CFAs, and its expression is mediated by RNA polymerase with sigma factor σ38. We generated a cfaB mutant of P. putida DOT-T1E and studied its response to solvents, acid pH and other stress conditions such as temperature changes, high osmolarity and the presence of antibiotics or heavy metals in the culture medium. A CfaB knockout mutant was more sensitive to solvent stress than the wild-type strain, but in contrast to Escherichia coli and Salmonella enterica, the P. putida cfaB mutant was as tolerant to acid shock as the wild-type strain. The cfaB mutant was also as tolerant as the parental strain to a number of drugs, antibiotics and other damaging agents.

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