• Open Access

Microbial cell immobilization

An annotated selection of World Wide Web sites relevant to the topics in Microbial Biotechnology

Authors

  • Lawrence P. Wackett

    1. McKnight Professor and Head
      Microbial Biochemistry and Biotechnology
      Department of Biochemistry
      Molecular Biology and Biophysics
      University of Minnesota
      St Paul, MN 55108, USA
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Cells immobilized for biosensor applications

http://scholar.lib.vt.edu/theses/available/etd-01032005-074039/unrestricted/Flemingthesis.pdf

This thesis describes several types of whole cell immobilization methods for the ultimate development of a whole-cell biosensor.

Immobilization and patterning of bacterial cells

http://pubs.acs.org/doi/abs/10.1021/la7038653

This web article describes a novel method for attaching bacterial cells to a surface. The cells can be oriented from lying down to standing up on the surface.

Immobilization of bacteria – patents

http://www.freshpatents.com/x1210601000psbc.php

This page contains an annotated list of patents describing different methods for immobilizing microbial cells and using the formulation for biocatalytic applications.

Living bacteria in silica gels

http://www.nature.com/nmat/journal/v1/n1/full/nmat709.html

This page describes an extensive study that examines the viability and physiology of microbial cells entrapped within silica gels. The viability exceeded that of cells free in a liquid suspension.

Bacterial growth of surface pili

http://medgadget.com/archives/2008/05/how_bacteria_grow_their_surface_pili.html

Pili are important in bacterial self-immobilization. This article summarizes new knowledge on the structure of bacterial pili.

Bacterial surface appendages

http://www.cehs.siu.edu/fix/medmicro/genmicr.htm

This general microbiology page describes bacterial surface structures relevant to cell self-immobilization.

Protease production by immobilized bacteria

http://www.aapspharmscitech.org/view.asp?art=pt060348

Bacteria entrapped within matrices are useful for the production and catalytic action of enzymes within the cell. This example describes the bacterial production of an alkaline protease.

Industrial application of immobilized cells

http://www.informapharmascience.com/doi/ref/10.3109/07388558309084659

This web article contains a large list of references describing application of microbial immobilized cells in biotechnology.

Bacteria attached to basalt

http://www.als.lbl.gov/als/compendium/AbstractManager/uploads/00114.pdf

In nature, microbes attach strongly to mineral surfaces. This article describes the constraints on microbial attachment to minerals.

Cell immobilization in hollow fiber membranes

http://www.eng.nus.edu.sg/EResnews/9705/may97p9.html

Hollow fiber membranes used for immobilization generally consist of dense and porous surfaces. Cells gain entry through the porous surface.

High efficiency bioconversion surface materials

http://www.faqs.org/patents/app/20090035599

This page describes a patent for bonding microbes to various surfaces that allow for maximum diffusion of substrates and, hence, high conversion rates.

Immobilized Arthrobacter degrade pentane

http://www.eugris.info/displayresource.asp?ResourceID=5585&Cat=document\

This web abstract describes a very stable immobilized cell preparation for the degradation of the solvent pentane.

Biogranulation: Self-immobilized bacteria

http://www.barc.ernet.in/webpages/letter/2005/200503-1.pdf

This web article reviews cell immobilization with an emphasis on bacterial self-immobilization for remediation of hazardous compounds.

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