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Ribose utilization by the human commensal Bifidobacterium breve UCC2003
Article first published online: 15 OCT 2009
DOI: 10.1111/j.1751-7915.2009.00152.x
© 2009 The Authors. Journal compilation © 2009 Society for Applied Microbiology and Blackwell Publishing Ltd
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Additional Information
How to Cite
Pokusaeva, K., Neves, A. R., Zomer, A., O'Connell-Motherway, M., MacSharry, J., Curley, P., Fitzgerald, G. F. and Van Sinderen, D. (2010), Ribose utilization by the human commensal Bifidobacterium breve UCC2003. Microbial Biotechnology, 3: 311–323. doi: 10.1111/j.1751-7915.2009.00152.x
Publication History
- Issue published online: 20 APR 2010
- Article first published online: 15 OCT 2009
- Received 27 May, 2009; revised 4 August, 2009; accepted 3 September, 2009.
Summary
Growth of Bifidobacterium breve UCC2003 on ribose leads to the transcriptional induction of the rbsACBDK gene cluster. Generation and phenotypic analysis of an rbsA insertion mutant established that the rbs gene cluster is essential for ribose utilization, and that its transcription is likely regulated by a LacI-type regulator encoded by rbsR, located immediately upstream of rbsA. Gel mobility shift assays using purified RbsRHis indicate that the promoter upstream of rbsABCDK is negatively controlled by RbsRHis binding to an 18 bp inverted repeat and that RbsRHis binding activity is modulated by d-ribose. The rbsK gene of the rbs operon of B. breve UCC2003 was shown to specify a ribokinase (EC 2.7.1.15), which specifically directs its phosphorylating activity towards d-ribose, converting this pentose sugar to ribose-5-phosphate.