• Open Access

Life cycle analysis of the model organism Rhodopirellula baltica SH 1T by transcriptome studies

Authors

  • Patricia Wecker,

    1. Max Planck Institute for Marine Microbiology, Microbial Genomics Group, Celsiusstr. 1, 28359 Bremen, Germany.
    2. Jacobs University Bremen gGmbH, Campusring 1, 28759 Bremen, Germany.
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    • Both authors contributed equally to this paper.

  • Christine Klockow,

    1. Max Planck Institute for Marine Microbiology, Microbial Genomics Group, Celsiusstr. 1, 28359 Bremen, Germany.
    2. Jacobs University Bremen gGmbH, Campusring 1, 28759 Bremen, Germany.
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    • Both authors contributed equally to this paper.

  • Margarete Schüler,

    1. Max Planck Institute for Biochemistry, Department. Molecular Structural Biology, Am Klopferspitz 18, 82152 Martinsried, Germany.
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  • Jérôme Dabin,

    1. UPMC Univ Paris 6, UMR 7139 Végétaux marins et Biomolécules, Station Biologique, F 29682, Roscoff, Bretagne, France.
    2. CNRS, UMR 7139 Végétaux marins et Biomolécules, Station Biologique, F 29682, Roscoff, Bretagne, France.
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  • Gurvan Michel,

    1. UPMC Univ Paris 6, UMR 7139 Végétaux marins et Biomolécules, Station Biologique, F 29682, Roscoff, Bretagne, France.
    2. CNRS, UMR 7139 Végétaux marins et Biomolécules, Station Biologique, F 29682, Roscoff, Bretagne, France.
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  • Frank O. Glöckner

    Corresponding author
    1. Max Planck Institute for Marine Microbiology, Microbial Genomics Group, Celsiusstr. 1, 28359 Bremen, Germany.
    2. Jacobs University Bremen gGmbH, Campusring 1, 28759 Bremen, Germany.
      E-mail fog@mpi-bremen.de; Tel. (+49) 2028 970; Fax (+49) 2028 580.
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E-mail fog@mpi-bremen.de; Tel. (+49) 2028 970; Fax (+49) 2028 580.

Summary

The marine organism Rhodopirellula baltica is a representative of the globally distributed phylum Planctomycetes whose members exhibit an intriguing lifestyle and cell morphology. The analysis of R. baltica's genome has revealed many biotechnologically promising features including a set of unique sulfatases and C1-metabolism genes. Salt resistance and the potential for adhesion in the adult phase of the cell cycle were observed during cultivation. To promote the understanding of this model organism and to specify the functions of potentially useful genes, gene expression throughout a growth curve was monitored using a whole genome microarray approach. Transcriptional profiling suggests that a large number of hypothetical proteins are active within the cell cycle and in the formation of the different cell morphologies. Numerous genes with potential biotechnological applications were found to be differentially regulated, revealing further characteristics of their functions and regulation mechanisms. More specifically, the experiments shed light on the expression patterns of genes belonging to the organism's general stress response, those involved in the reorganization of its genome and those effecting morphological changes. These transcriptomic results contribute to a better understanding of thus far unknown molecular elements of cell biology. Further, they pave the way for the biotechnological exploitation of R. baltica's distinctive metabolic features as a step towards sourcing the phylum Planctomycetes at large.

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