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Fig. S1. Results of the preliminary experiment for determining the maximum time for microcosm incubation. Solid squares represent DAPI counts; open squares represent FISH counts with probes EUB I-III; grey circles represent plate counts in marine agar. Standard errors of two replicate microcosms are shown.

Fig. S2. Variation in the percentage of prokaryotic cells hybridizing with probes EUBI-III (with respect to total DAPI counts) during microcosm incubations. Square symbols with solid lines correspond to winter microcosms. Circle symbols and dashed lines correspond to summer microcosms. Closed symbols correspond to controls and open symbols to diesel treatment.

Fig. S3. Variation in the relative abundance of T-RFs representative of Bacteroidetes. The sum of the relative abundances of two T-RFs obtained with the enzyme HhaI corresponding to sizes 92 and 94 nt are shown. Square symbols with solid lines correspond to winter microcosms. Circle symbols with dashed lines correspond to summer microcosms. Closed symbols correspond to controls and open symbols to diesel treatment.

Table S1. Detailed information on the results of the screening of clone libraries by RFLP analysis with enzyme HhaI.

Table S2. Detailed information on the accession numbers and affiliation of the clone sequences generated in this study.

Please note: Wiley-Blackwell are not responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article.

FilenameFormatSizeDescription
MBT_192_sm_fS1.gif34KSupporting info item
MBT_192_sm_fS2.gif19KSupporting info item
MBT_192_sm_fS3.gif18KSupporting info item
MBT_192_sm_tS1-2.doc350KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.