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Exopolysaccharides of Lactobacillus rhamnosus GG form a protective shield against innate immune factors in the intestine

  1. Sarah Lebeer*,
  2. Ingmar J. J. Claes,
  3. Tine L. A. Verhoeven,
  4. Jos Vanderleyden,
  5. Sigrid C. J. De Keersmaecker

Article first published online: 16 AUG 2010

DOI: 10.1111/j.1751-7915.2010.00199.x

Issue

Microbial Biotechnology

Microbial Biotechnology

Special Issue: Lactic Acid Bacteria. Editors: Michiel Kleerebezem and Willem M. de Vos.

Volume 4, Issue 3, pages 368–374, May 2011

Total views since publication: 220

Additional Information

How to Cite

Lebeer, S., Claes, I. J. J., Verhoeven, T. L. A., Vanderleyden, J. and De Keersmaecker, S. C. J. (2011), Exopolysaccharides of Lactobacillus rhamnosus GG form a protective shield against innate immune factors in the intestine. Microbial Biotechnology, 4: 368–374. doi: 10.1111/j.1751-7915.2010.00199.x

Author Information

  1. Centre of Microbial and Plant Genetics, K.U.Leuven, Kasteelpark Arenberg 20, B-3001 Leuven, Belgium.

* E-mail sarah.lebeer@biw.kuleuven.be; Tel. (+32) 16 321631; Fax (+32) 16 321966.

Publication History

  1. Issue published online: 25 APR 2011
  2. Article first published online: 16 AUG 2010
  3. Received 5 May, 2010; accepted 30 June, 2010.

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Reference

  • Alander, M., Satokari, R., Korpela, R., Saxelin, M., Vilpponen-Salmela, T., Mattila-Sandholm, T., and von Wright, A. (1999) Persistence of colonization of human colonic mucosa by a probiotic strain, Lactobacillus rhamnosus GG, after oral consumption. Appl Environ Microbiol 65: 351–354.
  • Andoh, A., Fujiyama, Y., Sakumoto, H., Uchihara, H., Kimura, T., Koyama, S., and Bamba, T. (1998) Detection of complement C3 and factor B gene expression in normal colorectal mucosa, adenomas and carcinomas. Clin Exp Immunol 111: 477–483.
    Direct Link:
  • van Baarlen, P., Troost, F.J., van Hemert, S., van der Meer, C., de Vos, W.M., de Groot, P.J., et al. (2009) Differential NF-kappa B pathways induction by Lactobacillus plantarum in the duodenum of healthy humans correlating with immune tolerance. Proc Natl Acad Sci USA 106: 2371–2376.
  • Boesten, R.J., and de Vos, W.M. (2008) Interactomics in the human intestine –Lactobacilli and Bifidobacteria make a difference. J Clin Gastroenterol 42: S163–S167.
  • Claesson, M.J., van Sinderen, D., and O'Toole, P.W. (2007) The genus Lactobacillus – a genomic basis for understanding its diversity. FEMS Microbiol Lett 269: 22–28.
    Direct Link:
  • Comstock, L.E., and Kasper, D.L. (2006) Bacterial glycans: key mediators of diverse host immune responses. Cell 126: 847–850.
  • Coyne, M.J., Chatzidaki, M., Paoletti, L., and Comstock, L.E. (2008) Role of glycan synthesis in colonization of the mammalian gut by the bacterial symbiont Bacteroides fragilis. Proc Natl Acad Sci USA 105: 13099–13104.
  • Dann, S.M., and Eckmann, L. (2007) Innate immune defenses in the intestinal tract. Curr Opin Gastroenterol 23: 115–120.
  • De Keersmaecker, S.C.J., Braeken, K., Verhoeven, T.L.A., Perea Vélez, M., Lebeer, S., Vanderleyden, J., and Hols, P. (2006) Flow cytometric testing of green fluorescent protein-tagged Lactobacillus rhamnosus GG for response to defensins. Appl Environ Microbiol 72: 4923–4930.
  • Di Caro, S., Tab, H., Grillo, A., Elia, C., Gasbarrini, G., Sepulveda, A.R., and Gasbarrini, A. (2005) Effects of Lactobacillus GG on genes expression pattern in small bowel mucosa. Dig Liver Dis 37: 320–329.
  • Doron, S., Snydman, D.R., and Gorbach, S.L. (2005) Lactobacillus GG: bacteriology and clinical applications. Gastroenterol Clin North Am 34: 483–498.
  • Fletcher, C.M., Coyne, M.J., Bentley, D.L., Villa, O.F., and Comstock, L.E. (2007) Phase-variable expression of a family of glycoproteins imparts a dynamic surface to a symbiont in its human intestinal ecosystem. Proc Natl Acad Sci USA 104: 2413–2418.
  • Francius, G., Lebeer, S., Alsteens, D., Wildling, L., Gruber, H.J., Hols, P., et al. (2008) Detection, localization and conformational analysis of single polysaccharide molecules on live bacteria. ACS Nano 2: 1921–1929.
  • Gorbach, S.L. (1996) The discovery of Lactobacillus GG. Nutr Today 31: 2S–5S.
  • Gorbach, S.L., Chang, T.W., and Goldin, B. (1987) Successful treatment of relapsing Clostridium difficile colitis with Lactobacillus GG. Lancet 2: 1519.
  • Gryllos, I., Tran-Winkler, H.J., Cheng, M.F., Chung, H., Bolcome, R., 3th, Lu, W., et al. (2008) Induction of group A Streptococcus virulence by a human antimicrobial peptide. Proc Natl Acad Sci USA 105: 16755–16760.
  • Guandalini, S., Pensabene, L., Zikri, M.A., Dias, J.A., Casali, L.J., Hoekstra, H., et al. (2000) Lactobacillus GG administered in oral rehydration solution to children with acute diarrhea: a multicenter European trial. J Pediatr Gastroenterol Nutr 30: 54–60.
  • Hooper, L.V., and Gordon, J.I. (2001) Glycans as legislators of host–microbial interactions: spanning the spectrum from symbiosis to pathogenicity. Glycobiology 11: 1R–10R.
  • Kalliomäki, M., Salminen, S., Arvilommi, H., Kero, P., Koskinen, P., and Isolauri, E. (2001) Probiotics in primary prevention of atopic disease: a randomised placebo-controlled trial. Lancet 357: 1076–1079.
  • Kalliomäki, M., Salminen, S., Poussa, T., Arvilommi, H., and Isolauri, E. (2003) Probiotics and prevention of atopic disease: 4-year follow-up of a randomised placebo-controlled trial. Lancet 361: 1869–1871.
  • Kankainen, M., Paulin, L., Tynkkynen, S., von Ossowski, I., Reunanen, J., Partanen, P., et al. (2009) Comparative genomic analysis of Lactobacillus rhamnosus GG reveals pili containing a human-mucus binding protein. Proc Natl Acad Sci USA 106: 17193–17198.
  • Klaenhammer, T.R., Barrangou, R., Buck, B.L., Azcarate-Peril, M.A., and Altermann, E. (2005) Genomic features of lactic acid bacteria effecting bioprocessing and health. FEMS Microbiol Rev 29: 393–409.
    Direct Link:
  • Kleerebezem, M., Hols, P., Bernard, E., Rolain, T., Zhou, M.M., Siezen, R.J., and Bron, P.A. (2010) The extracellular biology of the lactobacilli. FEMS Microbiol Rev 34: 199–230.
    Direct Link:
  • Landersjö, C., Yang, Z.N., Huttunen, E., and Widmalm, G. (2002) Structural studies of the exopolysaccharide produced by Lactobacillus rhamnosus strain GG (ATCC 53103). Biomacromolecules 3: 880–884.
  • Lebeer, S., Verhoeven, T.L.A., Perea Vélez, M., Vanderleyden, J., and De Keersmaecker, S.C.J. (2007) Impact of environmental and genetic factors on biofilm formation by the probiotic strain Lactobacillus rhamnosus GG. Appl Environ Microbiol 73: 6768–6775.
  • Lebeer, S., Vanderleyden, J., and De Keersmaecker, S. (2008a) Genes and molecules of Lactobacillus supporting probiotic action. Microbiol Mol Biol Rev 72: 728–764.
  • Lebeer, S., Claes, I.J.J., Verhoeven, T.L.A., Shen, C., Lambrichts, I., Ceuppens, J.L., et al. (2008b) Impact of luxS and suppressor mutations on the gastrointestinal transit of Lactobacillus rhamnosus GG. Appl Environ Microbiol 74: 4711–4718.
  • Lebeer, S., Verhoeven, T.L.A., Francius, G., Schoofs, G., Lambrichts, I., Dufrene, Y.F., et al. (2009) Identification of a gene cluster for the biosynthesis of a long galactose-rich exopolysaccharide in Lactobacillus rhamnosus GG and functional analysis of the priming glycosyltransferase. Appl Environ Microbiol 75: 3554–3563.
  • Lerouge, I., and Vanderleyden, J. (2002) O-antigen structural variation: mechanisms and possible roles in animal/plant–microbe interactions. FEMS Microbiol Rev 26: 17–47.
    Direct Link:
  • Limura, M., Gallo, R.L., Hase, K., Miyamoto, Y., Eckmann, L., and Kagnoff, M.F. (2005) Cathelicidin mediates innate intestinal defense against colonization with epithelial adherent bacterial pathogens. J Immunol 174: 4901–4907.
  • Liu, C.H., Lee, S.M., VanLare, J.M., Kasper, D.L., and Mazmanian, S.K. (2008) Regulation of surface architecture by symbiotic bacteria mediates host colonization. Proc Natl Acad Sci USA 105: 3951–3956.
  • Magee, A.D., and Yother, J. (2001) Requirement for capsule in colonization by Streptococcus pneumoniae. Infect Immun 69: 3755–3761.
  • Makarova, K., Slesarev, A., Wolf, Y., Sorokin, A., Mirkin, B., Koonin, E., et al. (2006) Comparative genomics of the lactic acid bacteria. Proc Natl Acad Sci USA 103: 15611–15616.
  • Marco, M.L., Pavan, S., and Kleerebezem, M. (2006) Towards understanding molecular modes of probiotic action. Curr Opin Biotechnol 17: 204–210.
  • Perea Vélez, M., Verhoeven, T.L.A., Draing, C., Von Aulock, S., Pfitzenmaier, M., Geyer, A., et al. (2007) Functional analysis of d-alanylation of lipoteichoic acid in the probiotic strain Lactobacillus rhamnosus GG. Appl Environ Microbiol 73: 3595–3604.
  • Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY, USA: Cold Spring Harbor Laboratory.
  • Sanders, M.E. (2008) Probiotics: definition, sources, selection, and uses. Clin Infect Dis 46: S58–S61.
  • Schneider, J.J., Unholzer, A., Schaller, M., Schafer-Korting, M., and Korting, H.C. (2005) Human defensins. J Mol Med 83: 587–595.
  • Tuomola, E., Crittenden, R., Playne, M., Isolauri, E., and Salminen, S. (2001) Quality assurance criteria for probiotic bacteria. Am J Clin Nutr 73: 393S–398S.
  • Vanderhoof, J.A., Whitney, D.B., Antonson, D.L., Hanner, T.L., Lupo, J.V., and Young, R.J. (1999) Lactobacillus GG in the prevention of antibiotic-associated diarrhea in children. J Pediatr 135: 564–568.
  • Weidenmaier, C., Kristian, S.A., and Peschel, A. (2003) Bacterial resistance to antimicrobial host defenses – an emerging target for novel antiinfective strategies? Curr Drug Targets 4: 643–649.
  • Zipfel, P.F., and Skerka, C. (2009) Complement regulators and inhibitory proteins. Nat Rev Immunol 9: 729–740.
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