Relevant uses of surface proteins – display on self-organized biological structures
Article first published online: 9 SEP 2011
© 2011 Massey University. Microbial Biotechnology © 2011 Society for Applied Microbiology and Blackwell Publishing Ltd
Special Issue: Microbial Vaccines and Immunomodulators. Guest Editors: Carlos A. Guzman, Ennio De Gregorio, Jan ter Meulen, and Martin Friede
Volume 5, Issue 2, pages 188–202, March 2012
How to Cite
Jahns, A. C. and Rehm, B. H. A. (2012), Relevant uses of surface proteins – display on self-organized biological structures. Microbial Biotechnology, 5: 188–202. doi: 10.1111/j.1751-7915.2011.00293.x
- Issue published online: 20 FEB 2012
- Article first published online: 9 SEP 2011
- Received 30 May, 2011; revised 12 July, 2011; accepted 18 July, 2011.
Proteins are often found attached to surfaces of self-assembling biological units such as whole microbial cells or subcellular structures, e.g. intracellular inclusions. In the last two decades surface proteins were identified that could serve as anchors for the display of foreign protein functions. Extensive protein engineering based on structure–function data enabled efficient display of technically and/or medically relevant protein functions. Small size, diversity of the anchor protein as well as support structure, genetic manipulability and controlled cultivation of phages, bacterial cells and yeasts contributed to the establishment of designed and specifically functionalized tools for applications as sensors, catalysis, biomedicine, vaccine development and library-based screening technologies. Traditionally, phage display is employed for library screening but applications in biomedicine and vaccine development are also perceived. For some diagnostic purposes phages are even too small in size so other carrier materials where needed and gave way for cell and yeast display. Only recently, intracellular inclusions such as magnetosomes, polyhydroxyalkanoate granules and lipid bodies were conceived as stable subcellular structures enabling the display of foreign protein functions and showing potential as specific and tailor-made devices for medical and biotechnological applications.