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Fig. S1. Overlap extension PCR to create EcoRI/BamHI sites at alkR–alkM DNA fragment.

Fig. S2. The effect of alkanes on bioluminescence expression. ADPWH_alk was activated by octadecane while the bioreporters ADPWH_lux (salicylate), ADPWH_Tol (toluene), ADPWH_recA (toxicity) cannot be activated by octadecane and the addition of octadecane did not increase bioluminescence expression.

Fig. S3. Biosensor ADPWH_lux growth curve in LB supplemented with 100 μM alkane with different carbon chain lengths.

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