• Open Access

In situ TCE degradation mediated by complex dehalorespiring communities during biostimulation processes

Authors

  • Eric Dugat-Bony,

    1. Clermont Université, Université Blaise Pascal, Laboratoire Microorganismes: Génome et Environnement, BP 10448, F63000, Clermont-Ferrand, France.
    2. UMR CNRS 6023, Université Blaise Pascal, 63000 Clermont-Ferrand, France.
    3. Clermont Université, Université d'Auvergne, Centre de Recherche en Nutrition Humaine Auvergne, EA 18, Conception, Ingénierie et Développement de l'Aliment et du Médicament, BP10448, F63000 Clermont-Ferrand, France.
    4. Clermont Université, Université d'Auvergne, UFR Pharmacie, Clermont-Ferrand, France.
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  • Corinne Biderre-Petit,

    1. Clermont Université, Université Blaise Pascal, Laboratoire Microorganismes: Génome et Environnement, BP 10448, F63000, Clermont-Ferrand, France.
    2. UMR CNRS 6023, Université Blaise Pascal, 63000 Clermont-Ferrand, France.
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  • Faouzi Jaziri,

    1. Clermont Université, Université Blaise Pascal, LIMOS, BP 10448, F-63000 Clermont-Ferrand, France.
    2. UMR CNRS 6158, LIMOS, F-63173 Aubière, France.
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  • Maude M. David,

    1. Environmental Microbial Genomics, Laboratoire Ampère, Ecole Centrale de Lyon, Université de Lyon, 69134 Ecully, France.
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  • Jérémie Denonfoux,

    1. Clermont Université, Université Blaise Pascal, Laboratoire Microorganismes: Génome et Environnement, BP 10448, F63000, Clermont-Ferrand, France.
    2. UMR CNRS 6023, Université Blaise Pascal, 63000 Clermont-Ferrand, France.
    3. Clermont Université, Université d'Auvergne, Centre de Recherche en Nutrition Humaine Auvergne, EA 18, Conception, Ingénierie et Développement de l'Aliment et du Médicament, BP10448, F63000 Clermont-Ferrand, France.
    4. Clermont Université, Université d'Auvergne, UFR Pharmacie, Clermont-Ferrand, France.
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  • Delina Y. Lyon,

    1. Environmental Microbial Genomics, Laboratoire Ampère, Ecole Centrale de Lyon, Université de Lyon, 69134 Ecully, France.
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  • Jean-Yves Richard,

    1. SITA Remediation (SUEZ Environnement company), 17 rue du périgord, 69330 MEYZIEU, France.
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  • Cyrille Curvers,

    1. SITA Remediation (SUEZ Environnement company), 17 rue du périgord, 69330 MEYZIEU, France.
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  • Delphine Boucher,

    1. UMR CNRS 6023, Université Blaise Pascal, 63000 Clermont-Ferrand, France.
    2. Clermont Université, Université d'Auvergne, Centre de Recherche en Nutrition Humaine Auvergne, EA 18, Conception, Ingénierie et Développement de l'Aliment et du Médicament, BP10448, F63000 Clermont-Ferrand, France.
    3. Clermont Université, Université d'Auvergne, UFR Pharmacie, Clermont-Ferrand, France.
    4. Clermont Université, Université d'Auvergne, Laboratoire Microorganismes: Génome et Environnement, BP 10448, F63000, Clermont-Ferrand, France.
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  • Timothy M. Vogel,

    1. Environmental Microbial Genomics, Laboratoire Ampère, Ecole Centrale de Lyon, Université de Lyon, 69134 Ecully, France.
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  • Eric Peyretaillade,

    1. UMR CNRS 6023, Université Blaise Pascal, 63000 Clermont-Ferrand, France.
    2. Clermont Université, Université d'Auvergne, Centre de Recherche en Nutrition Humaine Auvergne, EA 18, Conception, Ingénierie et Développement de l'Aliment et du Médicament, BP10448, F63000 Clermont-Ferrand, France.
    3. Clermont Université, Université d'Auvergne, UFR Pharmacie, Clermont-Ferrand, France.
    4. Clermont Université, Université d'Auvergne, Laboratoire Microorganismes: Génome et Environnement, BP 10448, F63000, Clermont-Ferrand, France.
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  • Pierre Peyret

    Corresponding author
    1. UMR CNRS 6023, Université Blaise Pascal, 63000 Clermont-Ferrand, France.
    2. Clermont Université, Université d'Auvergne, Centre de Recherche en Nutrition Humaine Auvergne, EA 18, Conception, Ingénierie et Développement de l'Aliment et du Médicament, BP10448, F63000 Clermont-Ferrand, France.
    3. Clermont Université, Université d'Auvergne, UFR Pharmacie, Clermont-Ferrand, France.
    4. Clermont Université, Université d'Auvergne, Laboratoire Microorganismes: Génome et Environnement, BP 10448, F63000, Clermont-Ferrand, France.
      E-mail pierre.peyret@u-clermont1.fr; Tel. (+33) 473177950; Fax (+33) 473275624.
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E-mail pierre.peyret@u-clermont1.fr; Tel. (+33) 473177950; Fax (+33) 473275624.

Summary

The bioremediation of chloroethene contaminants in groundwater polluted systems is still a serious environmental challenge. Many previous studies have shown that cooperation of several dechlorinators is crucial for complete dechlorination of trichloroethene to ethene. In the present study, we used an explorative functional DNA microarray (DechloArray) to examine the composition of specific functional genes in groundwater samples in which chloroethene bioremediation was enhanced by delivery of hydrogen-releasing compounds. Our results demonstrate for the first time that complete biodegradation occurs through spatial and temporal variations of a wide diversity of dehalorespiring populations involving both Sulfurospirillum, Dehalobacter, Desulfitobacterium, Geobacter and Dehalococcoides genera. Sulfurospirillum appears to be the most active in the highly contaminated source zone, while Geobacter was only detected in the slightly contaminated downstream zone. The concomitant detection of both bvcA and vcrA genes suggests that at least two different Dehalococcoides species are probably responsible for the dechlorination of dichloroethenes and vinyl chloride to ethene. These species were not detected on sites where cis-dichloroethene accumulation was observed. These results support the notion that monitoring dechlorinators by the presence of specific functional biomarkers using a powerful tool such as DechloArray will be useful for surveying the efficiency of bioremediation strategies.

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