Factors influencing the production of hydrogen by the purple non-sulphur phototrophic bacterium Rhodopseudomonas acidophila KU001
Article first published online: 18 APR 2012
© 2012 The Authors. Microbial Biotechnology © 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Volume 5, Issue 6, pages 674–678, November 2012
How to Cite
Merugu, R., Rudra, M. P. P., Badgu, N., Girisham, S. and Reddy, S. M. (2012), Factors influencing the production of hydrogen by the purple non-sulphur phototrophic bacterium Rhodopseudomonas acidophila KU001. Microbial Biotechnology, 5: 674–678. doi: 10.1111/j.1751-7915.2012.00346.x
- Issue published online: 14 OCT 2012
- Article first published online: 18 APR 2012
- Received 30 September, 2011; revised 28 February, 2012; accepted 2 March, 2012.
Rhodopseudomonas acidophila KU001 was isolated from leather industry effluents and the effect of different cultural conditions on hydrogen production was studied. Anaerobic light induced more hydrogen production than anaerobic dark conditions. Growing cells produced more amounts of hydrogen between 96 and 144 h of incubation. Resting and growing cells preferred a pH of 6.0 ± 0.24 for hydrogen production. Succinate was the most preferred carbon source for the production of hydrogen while citrate was a poor source of carbon. Acetate and malate were also good carbon sources for hydrogen production under anaerobic light. Among the nitrogen sources, R. acidophila preferred ammonium chloride followed by urea for production of hydrogen. L-tyrosine was the least preferred nitrogen source by both growing and resting cells.