Funding Information We thank the Federal Ministry of Food, Agriculture, and Consumer Protection (BMELV) for financial support within the ERA-IB project ‘BioProChemBB’ and Volker F. Wendisch (University of Bielefeld) for providing the plasmid pVWEx1-glpFKD.
Glycerol as a substrate for aerobic succinate production in minimal medium with Corynebacterium glutamicum
Article first published online: 18 APR 2012
© 2012 The Authors. Microbial Biotechnology © 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Special Issue: The Corynebacterium Cell Factory
Volume 6, Issue 2, pages 189–195, March 2013
How to Cite
Litsanov, B., Brocker, M. and Bott, M. (2013), Glycerol as a substrate for aerobic succinate production in minimal medium with Corynebacterium glutamicum. Microbial Biotechnology, 6: 189–195. doi: 10.1111/j.1751-7915.2012.00347.x
- Issue published online: 15 FEB 2013
- Article first published online: 18 APR 2012
- Received 21 December, 2011; revised 10 March, 2012; accepted 13 March, 2012.
Corynebacterium glutamicum, an established microbial cell factory for the biotechnological production of amino acids, was recently genetically engineered for aerobic succinate production from glucose in minimal medium. In this work, the corresponding strains were transformed with plasmid pVWEx1-glpFKD coding for glycerol utilization genes from Escherichia coli. This plasmid had previously been shown to allow growth of C. glutamicum with glycerol as sole carbon source. The resulting strains were tested in minimal medium for aerobic succinate production from glycerol, which is a by-product in biodiesel synthesis. The best strain BL-1/pVWEx1-glpFKD formed 79 mM (9.3 g l−1) succinate from 375 mM glycerol, representing 42% of the maximal theoretical yield under aerobic conditions. A specific succinate production rate of 1.55 mmol g−1 (cdw) h−1 and a volumetric productivity of 3.59 mM h−1 were obtained, the latter value representing the highest one currently described in literature. The results demonstrate that metabolically engineered strains of C. glutamicum are well suited for aerobic succinate production from glycerol.