• Open Access

Glycerol as a substrate for aerobic succinate production in minimal medium with Corynebacterium glutamicum

Authors

  • Boris Litsanov,

    1. Institut für Bio- und Geowissenschaften, IBG-1: Biotechnologie, Forschungszentrum Jülich, 52425 Jülich, Germany.
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  • Melanie Brocker,

    1. Institut für Bio- und Geowissenschaften, IBG-1: Biotechnologie, Forschungszentrum Jülich, 52425 Jülich, Germany.
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  • Michael Bott

    Corresponding author
    1. Institut für Bio- und Geowissenschaften, IBG-1: Biotechnologie, Forschungszentrum Jülich, 52425 Jülich, Germany.
      E-mail m.bott@fz-juelich.de; Tel. (+49) 2461 613294; Fax (+49) 2461 612710.
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  • Funding Information We thank the Federal Ministry of Food, Agriculture, and Consumer Protection (BMELV) for financial support within the ERA-IB project ‘BioProChemBB’ and Volker F. Wendisch (University of Bielefeld) for providing the plasmid pVWEx1-glpFKD.

E-mail m.bott@fz-juelich.de; Tel. (+49) 2461 613294; Fax (+49) 2461 612710.

Summary

Corynebacterium glutamicum, an established microbial cell factory for the biotechnological production of amino acids, was recently genetically engineered for aerobic succinate production from glucose in minimal medium. In this work, the corresponding strains were transformed with plasmid pVWEx1-glpFKD coding for glycerol utilization genes from Escherichia coli. This plasmid had previously been shown to allow growth of C. glutamicum with glycerol as sole carbon source. The resulting strains were tested in minimal medium for aerobic succinate production from glycerol, which is a by-product in biodiesel synthesis. The best strain BL-1/pVWEx1-glpFKD formed 79 mM (9.3 g l−1) succinate from 375 mM glycerol, representing 42% of the maximal theoretical yield under aerobic conditions. A specific succinate production rate of 1.55 mmol g−1 (cdw) h−1 and a volumetric productivity of 3.59 mM h−1 were obtained, the latter value representing the highest one currently described in literature. The results demonstrate that metabolically engineered strains of C. glutamicum are well suited for aerobic succinate production from glycerol.

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