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  • Open Access

The presence and role of bacterial quorum sensing in activated sludge

Authors

  • Grace Chong,

    1. Centre for Marine Bio-Innovation, School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, Australia
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  • Onder Kimyon,

    1. Centre for Marine Bio-Innovation, School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, Australia
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  • Scott A. Rice,

    1. Centre for Marine Bio-Innovation, School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, Australia
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  • Staffan Kjelleberg,

    1. Centre for Marine Bio-Innovation, School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, Australia
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  • Mike Manefield

    Corresponding author
    • Centre for Marine Bio-Innovation, School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, Australia
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For correspondence. E-mail manefield@unsw.edu.au; Tel. +61 (2) 9385 1780; Fax +61 (2) 9385 1779.

Summary

Activated sludge used for wastewater treatment globally is composed of a high-density microbial community of great biotechnological significance. In this study the presence and purpose of quorum sensing via N-acylated-l-homoserine lactones (AHLs) in activated sludge was explored. The presence of N-heptanoyl-l-homoserine lactone in organic extracts of sludge was demonstrated along with activation of a LuxR-based AHL monitor strain deployed in sludge, indicating AHL-mediated gene expression is active in sludge flocculates but not in the bulk aqueous phase. Bacterial isolates from activated sludge were screened for AHL production and expression of phenotypes commonly but not exclusively regulated by AHL-mediated gene transcription. N-acylated-l-homoserine lactone and exoenzyme production were frequently observed among the isolates. N-acylated-l-homoserine lactone addition to sludge upregulated chitinase activity and an AHL- and chitinase-producing isolate closely related to Aeromonas hydrophila was shown to respond to AHL addition with upregulation of chitinase activity. N-acylated-l-homoserine lactones produced by this strain were identified and genes ahyI/R and chiA, encoding AHL production and response and chitinase activity respectively, were sequenced. These experiments provide insight into the relationship between AHL-mediated gene expression and exoenzyme activity in activated sludge and may ultimately create opportunities to improve sludge performance.

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