• Open Access

Participation of putative glycoside hydrolases SlgC1 and SlgC2 in the biosynthesis of streptolydigin in Streptomyces lydicus

Authors

  • Cristina Gómez,

    1. Departamento de Biología Funcional, Universidad de Oviedo, Oviedo, Spain
    2. Instituto Universitario de Oncología del Principado de Asturias (I.U.O.P.A), Universidad de Oviedo, Oviedo, Spain
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  • Dina H. Horna,

    1. Departamento de Biología Funcional, Universidad de Oviedo, Oviedo, Spain
    2. Instituto Universitario de Oncología del Principado de Asturias (I.U.O.P.A), Universidad de Oviedo, Oviedo, Spain
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  • Carlos Olano,

    1. Departamento de Biología Funcional, Universidad de Oviedo, Oviedo, Spain
    2. Instituto Universitario de Oncología del Principado de Asturias (I.U.O.P.A), Universidad de Oviedo, Oviedo, Spain
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  • Carmen Méndez,

    1. Departamento de Biología Funcional, Universidad de Oviedo, Oviedo, Spain
    2. Instituto Universitario de Oncología del Principado de Asturias (I.U.O.P.A), Universidad de Oviedo, Oviedo, Spain
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  • José A. Salas

    Corresponding author
    1. Instituto Universitario de Oncología del Principado de Asturias (I.U.O.P.A), Universidad de Oviedo, Oviedo, Spain
    • Departamento de Biología Funcional, Universidad de Oviedo, Oviedo, Spain
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For correspondence. E-mail jasalas@uniovi.es; Tel. (+34) 985 103652; Fax (+34) 985 103652.

Summary

Two genes of the streptolydigin gene cluster in Streptomyces lydicus cluster encode putative family 16 glycoside hydrolases. Both genes are expressed when streptolydigin is produced. Inactivation of these genes affects streptolydigin production when the microorganism is grown in minimal medium containing either glycerol or d-glucans as carbon source. Streptolydigin yields in S. lydicus were increased by overexpression of either slgC1 or slgC2.

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